Overview

  • Product name

  • Description

    Rabbit polyclonal to DR4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide aa 420-468 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O00220

  • Positive control

    • IHC-P; Rat uterus and colon tissue. Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab216662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/500.

Target

  • Function

    Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
  • Tissue specificity

    Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
  • Sequence similarities

    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apo2 antibody
    • CD261 antibody
    • Cytotoxic TRAIL receptor antibody
    • Death receptor 4 antibody
    • DR4 antibody
    • NF related apoptosis-inducing ligand receptor 1 antibody
    • TNF receptor superfamily member 10a antibody
    • TNF-related apoptosis-inducing ligand receptor 1 antibody
    • TNFRSF10A antibody
    • TR10A_HUMAN antibody
    • TRAIL receptor 1 antibody
    • TRAIL-R1 antibody
    • TRAILR 1 antibody
    • TRAILR1 antibody
    • Tumor necrosis factor receptor superfamily member 10A antibody
    • Tumor necrosis factor receptor superfamily member 10a variant 2 antibody
    • Tumor necrosis factor receptor superfamily, member 10a antibody
    see all

Images

  • Anti-DR4 antibody (ab216662) at 1/500 dilution + MCF-7 cell lysates

    Secondary
    Conjugated secondary antibody at 1/10000 dilution
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat uterus tissue labeling DR4 with ab216662 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling DR4 with ab216662 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling DR4 with ab216662 at 1/500 dilution overnight at 4°C followed by conjugation to the secondary antibody and DAB staining. Prior to addition of the primary antibody, antigen retrieval was carried out by boiling in sodium citrate buffer (pH6.0) for 15min.  Endogenous peroxidase was blocked by 3% hydrogen peroxide for 30 minutes and blocking buffer (normal goat serum) added at 37°C for 20 min.

References

ab216662 has not yet been referenced specifically in any publications.

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