Key features and details
- Rabbit polyclonal to DR4
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-DR4 antibody
See all DR4 primary antibodies
DescriptionRabbit polyclonal to DR4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide aa 420-468 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O00220
- IHC-P; Rat uterus and colon tissue. Human colon carcinoma tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
FunctionReceptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Tissue specificityWidely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
Sequence similaritiesContains 1 death domain.
Contains 3 TNFR-Cys repeats.
- Information by UniProt
- Apo2 antibody
- CD261 antibody
- Cytotoxic TRAIL receptor antibody
Anti-DR4 antibody (ab216662) at 1/500 dilution + MCF-7 cell lysates
Conjugated secondary antibody at 1/10000 dilution
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat uterus tissue labeling DR4 with ab216662 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colon tissue labeling DR4 with ab216662 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling DR4 with ab216662 at 1/500 dilution overnight at 4°C followed by conjugation to the secondary antibody and DAB staining. Prior to addition of the primary antibody, antigen retrieval was carried out by boiling in sodium citrate buffer (pH6.0) for 15min. Endogenous peroxidase was blocked by 3% hydrogen peroxide for 30 minutes and blocking buffer (normal goat serum) added at 37°C for 20 min.
ab216662 has not yet been referenced specifically in any publications.