Product nameAnti-DR5 antibody [EPR1659(2)]
See all DR5 primary antibodies
DescriptionRabbit monoclonal [EPR1659(2)] to DR5
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DR5 aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: O14763
- HepG2, HeLa and HT1080 cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab181846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 40, 48 kDa (predicted molecular weight: 47 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
Tissue specificityWidely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
Involvement in diseaseSquamous cell carcinoma of the head and neck
Sequence similaritiesContains 1 death domain.
Contains 3 TNFR-Cys repeats.
- Information by UniProt
- Fas like protein antibody
- Apoptosis inducing protein TRICK2A/2B antibody
- Apoptosis inducing receptor TRAIL R2 antibody
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling DR5 with ab181846 at 1/400 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
All lanes : Anti-DR5 antibody [EPR1659(2)] (ab181846) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT1080 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 40,48 kDa why is the actual band size different from the predicted?
Western blot analysis of HeLa cell lysate immunoprecipitated using ab181846 at 1/30 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG was used at 1/1500 dilution.
This product has been referenced in:
- Nazim UM & Park SY Luteolin sensitizes human liver cancer cells to TRAIL-induced apoptosis via autophagy and JNK-mediated death receptor 5 upregulation. Int J Oncol 54:665-672 (2019). Read more (PubMed: 30431076) »
- Byun HS et al. C-27-carboxylated oleanane triterpenoids up-regulate TRAIL DISC assembly via p38 MAPK and CHOP-mediated DR5 expression in human glioblastoma cells. Biochem Pharmacol 158:243-260 (2018). Read more (PubMed: 30359578) »