Overview

  • Product name

    Anti-DR5 antibody [EPR22276]
    See all DR5 primary antibodies
  • Description

    Rabbit monoclonal [EPR22276] to DR5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DR5 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: O14763

  • Positive control

    • WB: HepG2, HT-1080 and U-87 MG whole cell lysate. Doxorubicin (ab120629) treated HCT116 cell lysate. IHC-P: Human colon cancer and endometrium tissue. IP: DR5 IP in U-87 MG whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 47 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
    • Tissue specificity

      Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
    • Involvement in disease

      Squamous cell carcinoma of the head and neck
    • Sequence similarities

      Contains 1 death domain.
      Contains 3 TNFR-Cys repeats.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Fas like protein antibody
      • Apoptosis inducing protein TRICK2A/2B antibody
      • Apoptosis inducing receptor TRAIL R2 antibody
      • CD262 antibody
      • CD262 antigen antibody
      • Cytotoxic TRAIL receptor 2 antibody
      • Death domain containing receptor for TRAIL/Apo 2L antibody
      • Death receptor 5 antibody
      • DR5 antibody
      • KILLER antibody
      • KILLER/DR5 antibody
      • OTTHUMP00000123492 antibody
      • OTTHUMP00000123493 antibody
      • p53 regulated DNA damage inducible cell death receptor(killer) antibody
      • TNF related apoptosis inducing ligand receptor 2 antibody
      • TNF-related apoptosis-inducing ligand receptor 2 antibody
      • TNFRSF10B antibody
      • TR10B_HUMAN antibody
      • TRAIL R2 antibody
      • TRAIL receptor 2 antibody
      • TRAIL-R2 antibody
      • TRAILR2 antibody
      • TRICK2 antibody
      • TRICK2A antibody
      • TRICK2B antibody
      • TRICKB antibody
      • Tumor necrosis factor receptor like protein ZTNFR9 antibody
      • Tumor necrosis factor receptor superfamily member 10B antibody
      • Tumor necrosis factor receptor superfamily, member 10b antibody
      • ZTNFR9 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DR5 with ab230969 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human colon cancer cells (PMID: 23284732) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling DR5 with ab230969 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in endothelial cells of human endometrium (PMID: 22789540, 12874246, 15987601) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • All lanes : Anti-DR5 antibody [EPR22276] (ab230969) at 1/1000 dilution

      Lane 1 : Untreated HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
      Lane 2 : HCT116 treated with 0.5 µM doxorubicin (ab120629) for 24 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa


      Exposure time: 3 minutes


      The molecular weight observed is consistent with what has been described in the literature, the 40kDa band is a cleaved form (PMID: 20515924, 16297203).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-DR5 antibody [EPR22276] (ab230969) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 2 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate
      Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa



      The molecular weight observed is consistent with what has been described in the literature. The 40kDa band is a cleaved form (PMID: 20515924, 16297203).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lanes 1: 3 minutes; Lanes 2-3: 59 seconds.

    • DR5 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate with ab230969 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230969 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
      Lane 1: U-87 MG whole cell lysate 10 µg (Input).
      Lane 2: ab230969 IP in U-87 MG whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230969 in U-87 MG whole cell lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 20 seconds.

      The molecular weight observed is consistent with what has been described in the literature. The 40kDa band is a cleaved form (PMID: 20515924, 16297203).

    References

    ab230969 has not yet been referenced specifically in any publications.

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