Product nameDR6 overexpression 293T lysate (whole cell)
DescriptionDR6 overexpression lysate
ab94106 is a 293T cell transfected lysate in which Human DR6 has been transiently over-expressed using a pCMV-DR6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: May activate NF-kappa-B and promote apoptosis. May activate JNK and be involved in T-cell differentiation. Required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N-terminal fragment of APP (N-APP). N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Tissue specificity: Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in adult liver and peripheral blood leukocytes. Similarity: Contains 1 death domain. Contains 4 TNFR-Cys repeats.
Our Abpromise guarantee covers the use of ab94106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94106 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-DR6 antibody (ab89770) at 1/500 dilution
Lane 1 :
DR6 overexpression 293T lysate (whole cell) (ab94106)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94106 has not yet been referenced specifically in any publications.