Key features and details
- Rabbit polyclonal to DRAGON
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DRAGON antibody
See all DRAGON primary antibodies
DescriptionRabbit polyclonal to DRAGON
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human DRAGON aa 219-467 (C terminal). NP_001012779
- 293T, HeLaS3, HepG2, Molt-4, Raji cell lysates. A431, H1299 whole cell lysates. U87 xenograft.
Protein previously labeled as Repulsive Guidance Molecule B.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionMember of the repulsive guidance molecule (RGM) family that contributes to the patterning of the developing nervous system (By similarity). Acts as a bone morphogenetic protein (BMP) coreceptor that potentiates BMP signaling (By similarity). Promotes neuronal adhesion (By similarity). May inhibit neurite outgrowth.
Sequence similaritiesBelongs to the repulsive guidance molecule (RGM) family.
Cellular localizationCell membrane. Membrane raft.
- Information by UniProt
- DRAGON antibody
- DRG11-responsive axonal guidance and outgrowth of neurite antibody
- Repulsive Guidance Molecule B antibody
All lanes : Anti-DRAGON antibody (ab96727) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ab96727 at 1/100 dilution.
ab96727 has not yet been referenced specifically in any publications.