Key features and details
- Rabbit polyclonal to DRAM - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DRAM antibody - N-terminal
See all DRAM primary antibodies
DescriptionRabbit polyclonal to DRAM - N-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human DRAM (N terminal). 16 amino acids. NP_060840.2.
Database link: Q8N682
- Human prostate tissue; Human Liver cells; K562 cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab188648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 26 kDa.|
|IHC-P||Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionLysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.
Sequence similaritiesBelongs to the DRAM/TMEM150 family.
Cellular localizationLysosome membrane.
- Information by UniProt
- Damage regulated autophagy modulator antibody
- Damage-regulated autophagy modulator 1 antibody
- Damage-regulated autophagy modulator antibody
Lane 1 : Anti-DRAM antibody - N-terminal (ab188648) at 0.5 µg/ml
Lane 2 : Anti-DRAM antibody - N-terminal (ab188648) at 1 µg/ml
Lane 3 : Anti-DRAM antibody - N-terminal (ab188648) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 26 kDa
Immunofluorescent analysis of Human Liver cells labeling DRAM with ab188648 at 20 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling DRAM with ab188648 at 3µg/ml.
ab188648 has been referenced in 1 publication.
- Wei M et al. DRAM1 deficiency affects the organization and function of the Golgi apparatus. Cell Signal 63:109375 (2019). PubMed: 31356858