Key features and details
- Rabbit polyclonal to DRAM2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DRAM2 antibody
See all DRAM2 primary antibodies
DescriptionRabbit polyclonal to DRAM2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse heart and liver tissue lysates. IHC-P: Human bladder cancer and heart tissue. ICC/IF: MCF7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionInduces apoptotic cell death when co-expressed with DRAM1.
Tissue specificityExpression is down-regulated in ovarian tumors (at protein level). Widely expressed with highest levels in placenta and heart. Not detected in brain or thymus.
Sequence similaritiesBelongs to the DRAM/TMEM150 family.
Cellular localizationLysosome membrane.
- Information by UniProt
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All lanes : Anti-DRAM2 antibody (ab230191) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Paraffin-embedded human bladder cancer tissue stained for DRAM2 with ab230191 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human heart tissue stained for DRAM2 with ab230191 at 1/100 dilution in immunohistochemical analysis.
MCF7 (human breast adenocarcinoma cell line) cells stained for DRAM2 (green) using ab230191 at 1/100 dilution in ICC/IF.
The secondary antibody is an AlexaFluor® 488-conjugated Goat anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230191 has not yet been referenced specifically in any publications.