• Product name
    DRAQ5™ (5mM)
  • Tested applications
    Suitable for: FM, Flow Cyt, ICC/IFmore details
  • General notes

    DRAQ5™ is a cell permeable far-red fluorescent DNA dye that can be used in fixed or non-fixed/ live cells in combination with common labels such as GFP or FITC.

    As with any cell­-permeant DNA intercalating probe, DRAQ5™may inhibit cell division in long-term assays and should be tested for any effect.

    DRAQ5™ can be used in flow cytometry, live cell imaging and cell-based assays and is highly compatible with existing protocols accross a wide range of instrumentation platforms.

    The chemical name of DRAQ5™ is 1, 5-bis{[2-(di-methylamino)ethyl]amino}-4, 8-dihydroxyanthracene-9, 10-dione.

    Key features of DRAQ5™ include:

    • Convenient ready-to-use aqueous solution
    • Rapid uptake into living cells, providing a high level of nuclear discrimination
    • No photobleaching effect
    • It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc.
    • No compensation needed with common FITC/GFP + PE combinations in flow cytometry
    • No RNase treatment required



    • 647 nm line optimal (Exmax 646 nm)
    • 488, 514, 568 and 633 nm lines, sub-optimal
    • Two-photon excitation (1047 nm) and excitation dark (700-850 nm)

    Emission (instrument dependent):

    • 665 nm to infra-red max 681 nm / 697 nm intercalated with dsDNA)
    • Minimal overlap with vis range e.g. GFP and FITC
    • Em. filters may include 695L, 715LP or 780 LP


    Multi-wavelength imaging with UV / vis fluorochromes

    • No photobleaching effect
    • No fluorescence enhancement upon DNA binding
    • Compatible with optics of benchtop flow, laser scanning cytometers and non-UV laser scanning and lamp-based confocal microscopes



Our Abpromise guarantee covers the use of ab108410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt 1/1000 - 1/250. For Gating of Nucleated cells = 5µM; For Cell Cycle Analysis = 20µM
ICC/IF 1/1000. For Cell-based assays, Immunofluorescence microscopy and In-Cell WB = 5µM
It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet section.



This product has been referenced in:
  • Lee JW  et al. The B cell death function of obinutuzumab-HDEL produced in plant (Nicotiana benthamiana L.) is equivalent to obinutuzumab produced in CHO cells. PLoS One 13:e0191075 (2018). Read more (PubMed: 29324849) »
  • Chou WC  et al. A Prognostic Model Based on Circulating Tumour Cells is Useful for Identifying the Poorest Survival Outcome in Patients with Metastatic Colorectal Cancer. Int J Biol Sci 14:137-146 (2018). Read more (PubMed: 29483832) »

See all 26 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

The information given in page 7 of protocol booklet states "DRAQ5™ staining is accelerated at 37°C and incubation time (5-30 minutes) may be reduced but this should be checked by titration and for ea...

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Thank you for your email. I am sorry for the inconvenience. Please check the following DRAQ5 specific publications;

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DRAQ5 In cell Western As Part of an Ab Trial scheme

Excellent Excellent 5/5 (Ease of Use)
DRAQ5 has been used to normalize for cell number( C42B cell line) . DRAQ5, a DNA dye has been used to label nuclei, in 1:1000 dilution in PBS, with a total volume of 50µl/ well. The cells were incubated for 30 minutes at room temperature in dark, before scanning using appropriate excitation (648 nm) and emission (705LP) filters. Cell number titration has been performed using 60000, 30000, 15000 and 7500, respectively. The fluorescence intensity of each well was quantified using ImageJ and plotted in a graph using GraphPrism, as demonstrated in following figure .

Dr. Dimitra Kalamida

Verified customer

Submitted Jun 05 2018

So good!

Good Good 4/5 (Ease of Use)
It is easy for staining with DAPI! so good.

Ms. Sehee Oh

Verified customer

Submitted Jan 31 2018

Vielen Dank für Ihre Anfrage.

Ich kann bestätigen, dass die DRAQ5 Färbung bei höheren Temperaturen verstärkt wird und deshalb die Inkubationszeit vielleicht angepasst werden muss. Je länger die Inkubationszeit desto mehr Signal erwarten w...

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1) We would expect the cell staining to diminish after a medium exchange. Despite DRAQ5’s high affinity for dsDNA there is still an equilibrium in place and this will be disturbed by removal of the low residual concentration in the culture medium.
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DRAQ5 co-staining

Excellent Excellent 5/5 (Ease of Use)
Compatible with FITC, Alexa 568, and Alexa 405 co-staining

Abcam user community

Verified customer

Submitted Dec 08 2014

DRAQ5 Abcam for In Cell Western Assay

Excellent Excellent 5/5 (Ease of Use)
Our lab uses DRAQ5 from Abcam for the In Cell Western Assay. DRAQ5 is used to provide accurate normalization over a broad range of cell densities. Specifically, it can be used for stoichiometric staining of DNA in live or fixed cells. DRAQ5 is used in the 700nm channel to normalize well-to well variation in cell number.
Picture shows In Cell Wester Assay using DRAQ5 in PC3 and LnCAP prostate cell lines.

Abcam user community

Verified customer

Submitted Jun 25 2014

Thank you for contacting us.

1. The most important consideration would be keep the cells in the presence of DRAQ5 and not to wash them. The nuclear stain should persist well but the likelihood is that progressively there would be ...

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ab108410 is not an antibody, it is a DNA dye and can be used on live or fixed cells. The blocking and permeabilization steps are optional. Therefore, you do not need to do them to get a good signal.

1-10 of 36 Abreviews or Q&A


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