DRAQ5 has been used to normalize for cell number( C42B cell line) . DRAQ5, a DNA dye has been used to label nuclei, in 1:1000 dilution in PBS, with a total volume of 50µl/ well. The cells were incubated for 30 minutes at room temperature in dark, before scanning using appropriate excitation (648 nm) and emission (705LP) filters. Cell number titration has been performed using 60000, 30000, 15000 and 7500, respectively. The fluorescence intensity of each well was quantified using ImageJ and plotted in a graph using GraphPrism, as demonstrated in following figure .
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DR. Dimitra Kalamida
Verified customer
Submitted Jun 05 2018