DRAQ5 has been used with LNCaP C4-2B cell line in flow cytometry for cell cycle analysis. The cells were scraped and centrifuged for 5 min at 4C at 1.200 rpm. Washed with cold PBS and further centrifuged for 5 min at 4C at 1.200 rpm. The cells were then stained with 200 ul of staining solution (DRAQ5 1:1000 dil in PBS) for 1 h at room temperature protected from lighted. The reaction volume was finally made up to 500 ul and the cells were examined by flow cytometry. There is a distinct difference in the apoptotic induced treated cells with a high apoptoptic percentage (M1) and lower M2, M3 and M4 percentages, representing G1, S and G2-M phases, respectively.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Dimitra Kalamida
Submitted Aug 22 2018