Overview

  • Product name
    DRAQ7™
  • Description
    DRAQ7™
  • Tested applications
    Suitable for: FM, Flow Cyt, ICC/IFmore details
  • General notes

    DRAQ7™ is a far-red fluorescent dye that only stains the nuclei in dead and permeabilized cells and can be used in combination with common labels such as GFP or FITC.

    DRAQ7 is the ideal tool to study dead or membrane-compromised cells because it does not enter intact, live cells. It is an ideal replacement for propidium iodide and 7-AAD, as is not excited by UV light and has no emission overlap with PE / PE homologues.

    Key features of DRAQ7 include:

    • Rapid staining of dsDNA/ nuclei of dead or permeabilized cells
    • Low photobleaching
    • It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc ...
    • Non-toxic in long-term culture
    • Can be combined with "live" dyes
    • No compensation needed with common FITC/GFP + PE combinations in flow cytometry
    • No wash or RNase treatment needed.

    SPECTRAL PROPERTIES

    Excitation:

    • 633 and 647 nm line optimal (Exmax 599 / 644 nm)
    • 488, 514 and 568 nm lines, sub-optimal (only by flow cytometry)

    Emission (instrument dependent):

    • 665 nm to infra-red max 678 nm / 697 nm intercalated with dsDNA)
    • Minimal overlap with vis range e.g. GFP and FITC
    • Em. filters may include 695L, 715LP or 780 LP

    Multi-wavelength imaging with UV / vis fluorochromes

    • No fluorescence enhancement upon DNA binding
    • Low photo-bleaching effect
    • Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluoroscence microscopes

Properties

Applications

Our Abpromise guarantee covers the use of ab109202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt 1/100. (final concentration = 3µM)
ICC/IF 1/100. (final concentration = 3µM)
It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet.

Images

  • Jurkat cells exposed to 1µM staurosporine for 24 hours show DRAQ7™ staining (top half of the plot). These cells have compromised membranes that allow entry of DRAQ7™ in the cells.
  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 μm thickness

    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody: Goat anti-rabbit IgG - H&L (AMCA) (ab123435)

    Nuclei were counterstained stained with DRAQ7™ (ab109202)

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 μm thickness


    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:200
    F-actin was stained with
    CytoPainter F-actin staining kit (blue) (ab112124), 1:1000
    Nuclei were counterstained stained with DRAQ7TM, 1:1000

Protocols

References

This product has been referenced in:
  • Perdijk O  et al. Sialyllactose and Galactooligosaccharides Promote Epithelial Barrier Functioning and Distinctly Modulate Microbiota Composition and Short Chain Fatty Acid Production In Vitro. Front Immunol 10:94 (2019). Read more (PubMed: 30809221) »
  • Cheng SY  et al. Anti-Invasion and Antiangiogenic Effects of Stellettin B through Inhibition of the Akt/Girdin Signaling Pathway and VEGF in Glioblastoma Cells. Cancers (Basel) 11:N/A (2019). Read more (PubMed: 30769863) »
See all 14 Publications for this product

Customer reviews and Q&As

Filter by Ratings

Human colon carcinoma cells stained with DRAQ7

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The cells were fixed with 4% PFA and permeabilized with 0.5% Triton. They were stained with DRAQ7 diluted 1:100 in PBS.

Dr. Eleni Petsalaki

Verified customer

Submitted Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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