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Can you please help with an enquiry we have received from a customer;
DRAQ7™ 1ml (0.3mM) (ab109202)
I would like to get some recommendation for a compatible fluorescent dye to stain all (live and dead) cells to be used together with DRAQ7 (to stain dead cells) for live or fixed tissues, thanks. I would appreciate a protocol too if it has been tested previously. Thanks.
Thanks for your help
Asked on Jan 14 2013
Thank you for contacting us.
We have several products from the CytoPainter range which may be of interest to your customer. These can be accessed from the following link:
CytoPainter staining kits | Abcam
These kits allow for the effectively visualization of cellular components and cellular tracking. If the customer is particularly interested in staining both live and dead cells, I would suggest the F-actin CytoPainter kit may be the most suitable. This selectively binds to F-actin and is suitable for fixed tissue sections or cell culture. The kit is available in 4 colours:
https://www.abcam.com/CytoPainter-F-actin-Staining-Kit-Blue-Fluorescence-ab112124.html: Blue (Ex/Em nm 350/450)
https://www.abcam.com/CytoPainter-F-actin-Staining-Kit-Green-Fluorescence-ab112125.html: Green (Ex/Em nm 500/520)
https://www.abcam.com/CytoPainter-F-actin-Staining-Kit-Orange-Fluorescence-ab112126.html: Orange (Ex/Em nm 550/575)
https://www.abcam.com/CytoPainter-F-actin-Staining-Kit-Red-Fluorescence-ab112127.html Red (Ex/Em nm 594/610)
We also have a number of apoptosis assay kits which may be of interest to the customer which can be accessed from the following link:
I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.
Answered on Jan 14 2013