For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
phone call in German:
Is it possible to stain the cells with ab109202 for a live /dead discrimination and then afterwards fix and permeabilise them for futher ICC/ Flow measuerments while still being able to discrimiate between what has been live and dead before the fixation?
Does DRAQ7 survive the fixing and permeabilisation?
Asked on Jul 14 2014
Ich habe nun vom Labor eine Antwort erhalten und kopiere diese im Original für Sie:
"In brief, it is likely that there will be significant washout of the DRAQ7 from the leaky cells into the previously viable ones when performing the multiple steps of subsequent IF staining, that is, after fixation.
This does not happen when the endpoint is just fixation with Formaldehyde and retaining this in the sample at 1% preserves the differential between leaky and intact cells (eg with a FP reporter and/or Hoechst for all cells), and good for extended periods ( >24 h)."
Zusammenfassend können wir DRAQ7 nicht empfehlen, wenn nach der Fixierung noch weiter Färbeschritte durchgeführt werden.
Anja HoffmannAbcam Scientific Support
Answered on Jul 14 2014