Overview

  • Product name

  • Description

    Rabbit polyclonal to DRD4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human DRD4 aa 380-410 (C terminal).
    Database link: P21917

  • Positive control

    • RAW 264.7 cell lysate.
  • General notes

     This product was previously labelled as Dopamine Receptor D4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab135978 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    Polyubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex: polyubiquitination does not lead to degradation of DRD4 protein.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • D(2C) dopamine receptor antibody
    • D(4) dopamine receptor (D(2C) dopamine receptor). antibody
    • D(4) dopamine receptor antibody
    • D4 dopamine receptor antibody
    • D4DR antibody
    • Dopamine D4 receptor antibody
    • DRD 4 antibody
    • Drd4 antibody
    • DRD4_HUMAN antibody
    see all

Images

  • Anti-DRD4 antibody (ab135978) at 1/500 dilution + RAW 264.7 cell lysate at 30 µg

    Predicted band size: 48 kDa

References

This product has been referenced in:

  • Zhu Y  et al. Dopamine D1 and D3 Receptors Modulate Heroin-Induced Cognitive Impairment through Opponent Actions in Mice. Int J Neuropsychopharmacol 20:257-268 (2017). Read more (PubMed: 27815417) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (K562)
Permeabilization
Yes - Life tech: fix & perm medium B
Gating Strategy
Live cells
Specification
K562
Fixation
Life tech: fix & perm medium A

Jake Ritchie

Verified customer

Submitted Sep 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult neurons)
Permeabilization
Yes - 0.2% Triton with 3%H2O2 and 10% methanol
Specification
Adult neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 03 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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