• Product name

  • Description

    Rabbit polyclonal to DRD4
  • Host species

  • Specificity

    Cross-reactivity: D4 Dopamine Receptor (176-185) 100% D4 Dopamine Receptor 60% D1 Dopamine Receptor (9-21) 0% D1 Dopamine Receptor 0% D2 Dopamine Receptor (272-282) 0% D2 Dopamine Receptor 0% D3 Dopamine Receptor (2-10) 0% D3 Dopamine Receptor 0% D5 Dopamine Receptor (23-35) 0% D5 Dopamine Receptor 0%
  • Tested applications

    Suitable for: ICC, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human DRD4 aa 176-185 (N terminal).


    (Peptide available as ab49539)

  • Positive control

    • Whole rat brain IF/ICC: PC12 cell line.
  • General notes

     This product was previously labelled as Dopamine Receptor D4




Our Abpromise guarantee covers the use of ab20424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10000.
WB 1/500. Detects a band of approximately 48-53 kDa (predicted molecular weight: 48 kDa).
ICC/IF 1/10000.
IHC-P Use at an assay dependent concentration. PubMed: 22155235


  • Function

    Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational

    Polyubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex: polyubiquitination does not lead to degradation of DRD4 protein.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • D(2C) dopamine receptor antibody
    • D(4) dopamine receptor (D(2C) dopamine receptor). antibody
    • D(4) dopamine receptor antibody
    • D4 dopamine receptor antibody
    • D4DR antibody
    • Dopamine D4 receptor antibody
    • DRD 4 antibody
    • Drd4 antibody
    • DRD4_HUMAN antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat gastric tissue labelling DRD4 with ab20424 at 1/1000. DRD4 was not found in the gastric mucosa (e - left) but was observed in the gastric enteric plexus (f - right). Paraffin sections were baked at 60°C for 2 hours, then deparaffinized in xylene for 20 minutes. Slides were rehydrated in descending concentrations of alcohol and transferred from 70% alcohol solution to distilled water for rinsing. Before staining, slides were pretreated with 10 mM citric acid (pH 6.0) at 92–98°C for 10 min and incubated in 10% normal serum and 0.1% Triton X-100 for 30 min. The sections were then incubated with the primary antibody, followed by the appropriate secondary antibody. Sections were washed in PBS and mounted in 90% glycerin PBS.

  • ab20424 stained PC12 cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20424 at 1/100) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.


This product has been referenced in:

  • Wang Q  et al. Cellular localization of dopamine receptors in the gastric mucosa of rats. Biochem Biophys Res Commun 417:197-203 (2012). IHC-P ; Rat . Read more (PubMed: 22155235) »
  • Krishnan B  et al. Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced long-term potentiation in the rat amygdala following cocaine withdrawal. Eur J Neurosci 31:1027-42 (2010). Read more (PubMed: 20377617) »
See all 2 Publications for this product

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