Key features and details
- Rabbit polyclonal to DRD5
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DRD5 antibody
See all DRD5 primary antibodies
DescriptionRabbit polyclonal to DRD5
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chicken
Synthetic peptide within Human DRD5 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P21918
- Human brain tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
This product was previously labelled as Dopamine Receptor D5
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab32620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Tissue specificityNeuron-specific, localized primarily within limbic regions of the brain.
Involvement in diseaseDefects in DRD5 are a cause of benign essential blepharospasm (BEB) [MIM:606798]. BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- D(1B) dopamine receptor antibody
- D(5) dopamine receptor antibody
- D1 beta dopamine receptor antibody
Ab32620, at a 1/50 dilution, staining DRD5 in formalin fixed paraffin embedded human brain tissue by Immunohistochemistry.
ICC/IF image of ab32620 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab32620 has not yet been referenced specifically in any publications.