Anti-DRD5 antibody (ab40656)
Key features and details
- Rabbit polyclonal to DRD5
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-DRD5 antibody
See all DRD5 primary antibodies -
Description
Rabbit polyclonal to DRD5 -
Host species
Rabbit -
Specificity
Cross-reacts with Human D5 Dopamine Receptor(Ac23-35) 100%, D5 Dopamine Receptor 80%. Does not cross react with other dopamine receptors (D1, D2, D3, D4).
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Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Human, Gorilla -
Immunogen
Synthetic peptide corresponding to Human DRD5 aa 23-35 (acetyl G23) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence: AcGNAVGGSAGAPP
Database link: P21918 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Rehydrate the lyophilized antibody with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer (such as 10 mg/ml BSA in PBS) for more dilute antibody. -
Storage instructions
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
Blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa. Blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
Target
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Function
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
Tissue specificity
Neuron-specific, localized primarily within limbic regions of the brain. -
Involvement in disease
Defects in DRD5 are a cause of benign essential blepharospasm (BEB) [MIM:606798]. BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1816 Human
- Entrez Gene: 25195 Rat
- Omim: 126453 Human
- SwissProt: P21918 Human
- SwissProt: P25115 Rat
- Unigene: 380681 Human
- Unigene: 138110 Rat
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Alternative names
- D(1B) dopamine receptor antibody
- D(5) dopamine receptor antibody
- D1 beta dopamine receptor antibody
see all
Datasheets and documents
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Datasheet download
References (2)
ab40656 has been referenced in 2 publications.
- Zhu Y et al. Dopamine D1 and D3 Receptors Modulate Heroin-Induced Cognitive Impairment through Opponent Actions in Mice. Int J Neuropsychopharmacol 20:257-268 (2017). PubMed: 27815417
- Reyes S et al. Variability in neuronal expression of dopamine receptors and transporters in the substantia nigra. Mov Disord 28:1351-9 (2013). PubMed: 23674405