Overview

  • Product name

  • Description

    Rabbit polyclonal to DREAM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human DREAM.

  • Positive control

    • Human brain cortex neurons for IHC-P; MCF-7, Jurkat and A549 cells for Western blot; and Hela cells for Immunofluorescence.
  • General notes

    Previously labelled as CSEN. 

Properties

Applications

Our Abpromise guarantee covers the use of ab133212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
ELISA 1/20000.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
    Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.
    May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation.
  • Tissue specificity

    Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.
  • Sequence similarities

    Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Post-translational
    modifications

    Palmitoylated. Palmitoylation enhances association with the plasma membrane.
    Proteolytically cleaved by caspase-3.
    Phosphorylation at Ser-63 inhibits cleavage by CASP3.
  • Cellular localization

    Cytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4933407H12Rik antibody
    • A type potassium channel modulatory protein 3 antibody
    • A-type potassium channel modulatory protein 3 antibody
    • AI413860 antibody
    • Calsenilin antibody
    • CSEN antibody
    • CSEN_HUMAN antibody
    • DRE antagonist modulator antibody
    • DRE-antagonist modulator antibody
    • DREAM antibody
    • EF hand transcription factor antibody
    • KCHIP 3 antibody
    • KChIP3 antibody
    • KCNIP 3 antibody
    • Kcnip3 antibody
    • Kv channel interacting protein 3 antibody
    • Kv channel-interacting protein 3 antibody
    • MGC18289 antibody
    • Potassium channel interacting protein 3 antibody
    • R74849 antibody
    see all

Images

  • All lanes : Anti-DREAM antibody (ab133212) at 1/500 dilution

    Lane 1 : Extract from MCF-7 cells
    Lane 2 : Extract from Jurkat cells
    Lane 3 : Extract from A549 cells
    Lane 4 : Extract from Jurkat cells with immunizing peptide

    Predicted band size: 29 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex neurons labelling DREAM with ab133212 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

  • Immunofluorescent analysis of Hela cells labelling DREAM with ab133212 at 1/100 dilution (left). Right panel is treated with the immunizing peptide.

References

This product has been referenced in:

  • You X  et al. Endogenous hydrogen sulfide contributes to uterine quiescence during pregnancy. Reproduction 153:535-543 (2017). Read more (PubMed: 28188160) »
  • Hu TX  et al. MiR 20a,-20b and -200c are involved in hydrogen sulfide stimulation of VEGF production in human placental trophoblasts. Placenta 39:101-10 (2016). Read more (PubMed: 26992682) »
See all 2 Publications for this product

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