Product nameAnti-DREAM antibody
See all DREAM primary antibodies
DescriptionRabbit polyclonal to DREAM
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human DREAM.
- Human brain cortex neurons for IHC-P; MCF-7, Jurkat and A549 cells for Western blot; and Hela cells for Immunofluorescence.
Previously labelled as CSEN.
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 48% PBS, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 29 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionCalcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.
May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation.
Tissue specificityHighly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.
Sequence similaritiesBelongs to the recoverin family.
Contains 4 EF-hand domains.
modificationsPalmitoylated. Palmitoylation enhances association with the plasma membrane.
Proteolytically cleaved by caspase-3.
Phosphorylation at Ser-63 inhibits cleavage by CASP3.
Cellular localizationCytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.
- Information by UniProt
- 4933407H12Rik antibody
- A type potassium channel modulatory protein 3 antibody
- A-type potassium channel modulatory protein 3 antibody
All lanes : Anti-DREAM antibody (ab133212) at 1/500 dilution
Lane 1 : Extract from MCF-7 cells
Lane 2 : Extract from Jurkat cells
Lane 3 : Extract from A549 cells
Lane 4 : Extract from Jurkat cells with immunizing peptide
Predicted band size: 29 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex neurons labelling DREAM with ab133212 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunofluorescent analysis of Hela cells labelling DREAM with ab133212 at 1/100 dilution (left). Right panel is treated with the immunizing peptide.
This product has been referenced in:
- You X et al. Endogenous hydrogen sulfide contributes to uterine quiescence during pregnancy. Reproduction 153:535-543 (2017). Read more (PubMed: 28188160) »
- Hu TX et al. MiR 20a,-20b and -200c are involved in hydrogen sulfide stimulation of VEGF production in human placental trophoblasts. Placenta 39:101-10 (2016). Read more (PubMed: 26992682) »