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Anti-DREAM antibody (ab3474)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (ab3474)

    Key features and details

    • Rabbit polyclonal to DREAM
    • Suitable for: ICC/IF
    • Reacts with: Rabbit, Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human DREAM protein (ab101211)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-DREAM antibody
      See all DREAM primary antibodies
    • Description

      Rabbit polyclonal to DREAM
    • Host species

      Rabbit
    • Specificity

      Detects KChIP 3 from rabbit cerebellar granule cells.
    • Tested Applications & Species

      Application Species
      ICC/IF
      Rabbit
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Mouse DREAM aa 30-45.
      Sequence:

      IKWQRPRFTRQALMRC


      (Peptide available as ab5866)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Previously labelled as CSEN. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Other
      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • More Ion Channels
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Calcium Channels

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human DREAM protein (ab101211)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3474 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Rabbit
    All applications
    Mouse
    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.

    Target

    • Function

      Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
      Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.
      May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation.
    • Tissue specificity

      Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.
    • Sequence similarities

      Belongs to the recoverin family.
      Contains 4 EF-hand domains.
    • Post-translational
      modifications

      Palmitoylated. Palmitoylation enhances association with the plasma membrane.
      Proteolytically cleaved by caspase-3.
      Phosphorylation at Ser-63 inhibits cleavage by CASP3.
    • Cellular localization

      Cytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.
    • Target information above from: UniProt accession Q9Y2W7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 30818 Human
      • Entrez Gene: 56461 Mouse
      • Omim: 604662 Human
      • SwissProt: Q9Y2W7 Human
      • SwissProt: Q9QXT8 Mouse
      • Unigene: 437376 Human
      • Unigene: 315292 Mouse
      • Alternative names

        • 4933407H12Rik antibody
        • A type potassium channel modulatory protein 3 antibody
        • A-type potassium channel modulatory protein 3 antibody
        • AI413860 antibody
        • Calsenilin antibody
        • CSEN antibody
        • CSEN_HUMAN antibody
        • DRE antagonist modulator antibody
        • DRE-antagonist modulator antibody
        • DREAM antibody
        • EF hand transcription factor antibody
        • KCHIP 3 antibody
        • KChIP3 antibody
        • KCNIP 3 antibody
        • Kcnip3 antibody
        • Kv channel interacting protein 3 antibody
        • Kv channel-interacting protein 3 antibody
        • MGC18289 antibody
        • Potassium channel interacting protein 3 antibody
        • R74849 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (ab3474)
        Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (ab3474)

        Immunolocalization of DREAM in Rabbit granule cells using ab3474.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab3474? Please let us know so that we can cite the reference in this datasheet.

      ab3474 has been referenced in 1 publication.

      • Tian NX  et al. KChIP3 N-Terminal 31-50 Fragment Mediates Its Association with TRPV1 and Alleviates Inflammatory Hyperalgesia in Rats. J Neurosci 38:1756-1773 (2018). PubMed: 29335353

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