Anti-DREAM antibody (ab3474)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-DREAM antibody
See all DREAM primary antibodies -
Description
Rabbit polyclonal to DREAM -
Host species
Rabbit -
Specificity
Detects KChIP 3 from rabbit cerebellar granule cells. -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Rabbit, Human, Non human primates
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Mouse DREAM aa 30-45.
Sequence:IKWQRPRFTRQALMRC
(Peptide available asab5866) -
Positive control
- Immunohistochemical staining of KChIP3 in rabbit granule cells yields a punctate staining pattern at the plasma membrane.
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General notes
Previously labelled as CSEN.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab3474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/25. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.
May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation. -
Tissue specificity
Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease. -
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains. -
Post-translational
modificationsPalmitoylated. Palmitoylation enhances association with the plasma membrane.
Proteolytically cleaved by caspase-3.
Phosphorylation at Ser-63 inhibits cleavage by CASP3. -
Cellular localization
Cytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 30818 Human
- Entrez Gene: 56461 Mouse
- Entrez Gene: 65199 Rat
- Omim: 604662 Human
- SwissProt: Q9Y2W7 Human
- SwissProt: Q9QXT8 Mouse
- SwissProt: Q9JM47 Rat
- Unigene: 437376 Human
see all -
Alternative names
- 4933407H12Rik antibody
- A type potassium channel modulatory protein 3 antibody
- A-type potassium channel modulatory protein 3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (ab3474)Immunolocalization of DREAM in Rabbit granule cells using ab3474.
Datasheets and documents
References
This product has been referenced in:
- Tian NX et al. KChIP3 N-Terminal 31-50 Fragment Mediates Its Association with TRPV1 and Alleviates Inflammatory Hyperalgesia in Rats. J Neurosci 38:1756-1773 (2018). Read more (PubMed: 29335353) »
