Anti-DREAM antibody (ab3474)
Key features and details
- Rabbit polyclonal to DREAM
- Suitable for: ICC/IF
- Reacts with: Rabbit, Human
- Isotype: IgG
Overview
-
Product name
Anti-DREAM antibody
See all DREAM primary antibodies -
Description
Rabbit polyclonal to DREAM -
Host species
Rabbit -
Specificity
Detects KChIP 3 from rabbit cerebellar granule cells. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Rabbit
-
Immunogen
Synthetic peptide corresponding to Mouse DREAM aa 30-45.
Sequence:IKWQRPRFTRQALMRC
(Peptide available asab5866) -
General notes
Previously labelled as CSEN.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Rabbit
|
| All applications |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.
May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation. -
Tissue specificity
Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease. -
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains. -
Post-translational
modificationsPalmitoylated. Palmitoylation enhances association with the plasma membrane.
Proteolytically cleaved by caspase-3.
Phosphorylation at Ser-63 inhibits cleavage by CASP3. -
Cellular localization
Cytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 30818 Human
- Entrez Gene: 56461 Mouse
- Omim: 604662 Human
- SwissProt: Q9Y2W7 Human
- SwissProt: Q9QXT8 Mouse
- Unigene: 437376 Human
- Unigene: 315292 Mouse
-
Alternative names
- 4933407H12Rik antibody
- A type potassium channel modulatory protein 3 antibody
- A-type potassium channel modulatory protein 3 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-DREAM antibody (ab3474)
Immunolocalization of DREAM in Rabbit granule cells using ab3474.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab3474 has been referenced in 1 publication.
- Tian NX et al. KChIP3 N-Terminal 31-50 Fragment Mediates Its Association with TRPV1 and Alleviates Inflammatory Hyperalgesia in Rats. J Neurosci 38:1756-1773 (2018). PubMed: 29335353