Key features and details
- Rabbit polyclonal to DREAM
- Suitable for: ICC/IF, IHC-P, WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DREAM antibody
See all DREAM primary antibodies
DescriptionRabbit polyclonal to DREAM
Specificityab61770 detects endogenous levels of total DREAM protein
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human DREAM (phospho S63). (D-S-SP-D-S)
- Human brain tissue; extracts from A431 cells
Previously labelled as CSEN.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab61770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
FunctionCalcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.
May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation.
Tissue specificityHighly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.
Sequence similaritiesBelongs to the recoverin family.
Contains 4 EF-hand domains.
modificationsPalmitoylated. Palmitoylation enhances association with the plasma membrane.
Proteolytically cleaved by caspase-3.
Phosphorylation at Ser-63 inhibits cleavage by CASP3.
Cellular localizationCytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.
- Information by UniProt
- 4933407H12Rik antibody
- A type potassium channel modulatory protein 3 antibody
- A-type potassium channel modulatory protein 3 antibody
All lanes : Anti-DREAM antibody (ab61770) at 1/500 dilution
Lane 1 : extracts from A431 cells with no peptide
Lane 2 : extracts from A431 cells with peptide
Predicted band size: 29 kDa
Observed band size: 29 kDa
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61770 at a dilution of 1/50. left: untreated sample. Right: sample treated with immunising peptide.
ICC/IF image of ab61770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61770, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab61770 has been referenced in 1 publication.
- Zhu Z et al. Vasopressin regulates hypothalamic GnRH synthesis: Histomorphological evidence in hypothalamus and biological effects in GT1-7 cells. Life Sci 227:166-174 (2019). PubMed: 31026452