Key features and details
- Rabbit polyclonal to Drebrin
- Suitable for: ICC, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
Product nameAnti-Drebrin antibody
See all Drebrin primary antibodies
DescriptionRabbit polyclonal to Drebrin
Tested applicationsSuitable for: ICC, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Synthetic peptide conjugated to KLH, corresponding to amino acids 22-42 of Human Drebrin.
- HeLa cell line (ab7898). Rat e16 spinal cord.
General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Our Abpromise guarantee covers the use of ab11068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/1000. Predicted molecular weight: 95-120 kDa. ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations.|
FunctionDrebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
Tissue specificityBrain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 1 ADF-H domain.
- Information by UniProt
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
Rabbit polyclonal to Drebrin (ab11068) used in IHC(Formalin/PFA-fixed paraffin-embedded sections; Standard stABCpx-DAB detection). ab11068 detecting Drebrin protein on Rat spinal cord E16 embryo sections fixed in formaldehyde. Antigen retrieval step: Heat mediated. 1% BSA as blocking agent for 10 mins at RT. Ab11068 was used at a dilution of 1/2500 incubated for 16 hours. Secondary Antibody is biotin labeled anti rabbit IgG (1/300). Drebrin positivity conformed to published localisation: essentially, many non-neuronal developing tissues were positive, eg: skeletal primary myotubes. This picture is of spinal cord floor plate region (x20). Nuclei were counterstained using Mayer's Haemalum.
ab11068 staining Drebrin in mouse COR-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton x100 and blocking with 2% BSA was done at 250C for 15 minutes. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.25% gelatin in PBS) for 3 hours at 25°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
ICC/IF image of ab11068 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab11068 has been referenced in 18 publications.
- Martinsson I et al. APP depletion alters selective pre- and post-synaptic proteins. Mol Cell Neurosci 95:86-95 (2019). PubMed: 30763689
- Schätzle P et al. Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry. Curr Biol 28:2081-2093.e6 (2018). PubMed: 29910073
- Klementieva O et al. Pre-plaque conformational changes in Alzheimer's disease-linked Aß and APP. Nat Commun 8:14726 (2017). IHC ; Mouse . PubMed: 28287086
- Trivedi N et al. Drebrin-mediated microtubule-actomyosin coupling steers cerebellar granule neuron nucleokinesis and migration pathway selection. Nat Commun 8:14484 (2017). IHC . PubMed: 28230156
- Kasioulis I et al. Inter-dependent apical microtubule and actin dynamics orchestrate centrosome retention and neuronal delamination. Elife 6:N/A (2017). PubMed: 29058679