Anti-Drebrin antibody (ab60933)
Key features and details
- Rabbit polyclonal to Drebrin
- Suitable for: ICC/IF, WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Drebrin antibody
See all Drebrin primary antibodies -
Description
Rabbit polyclonal to Drebrin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Quail -
Immunogen
Synthetic peptide corresponding to Human Drebrin aa 600 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab101313) -
Positive control
- WB: SH-SY5Y, SK N SH and SK N BE Whole Cell Lysate, Human, Rat and Mouse Cortex Neuronal cell lysate, Rat and Mouse Hippocampus Tissue Lysate and Malme 3M Whole Cell Lysate. ICC/IF: Primary rat neurons/glia, DIV14 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 71 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 71 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively. -
Tissue specificity
Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. -
Sequence similarities
Contains 1 ADF-H domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 396496 Chicken
- Entrez Gene: 505406 Cow
- Entrez Gene: 1627 Human
- Entrez Gene: 56320 Mouse
- Entrez Gene: 81653 Rat
- Omim: 126660 Human
- SwissProt: P18302 Chicken
- SwissProt: Q16643 Human
see all -
Alternative names
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Hu cerebellum whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab60933 observed at 100120 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab60933 was shown to specifically react with Drebrin in wild-type HAP1 cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. ab60933 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ab60933 staining Drebrin in primary rat neurons/glia, DIV14 (prepared from E18 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDHEP) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab60933 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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All lanes : Anti-Drebrin antibody (ab60933) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 4 : Human Cortex Neuronal cell lysate
Lane 5 : Rat Cortex Neuronal cell lysate
Lane 6 : Mouse Cortex Neuronal cell lysate
Lane 7 : Rat Hippocampus Tissue Lysate
Lane 8 : Mouse Hippocampus Tissue Lysate
Lane 9 : Malme 3M (Human melanoma cells) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 100,120 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
The banding pattern observed is consistent with what has been described in the literature (PMID:10234022, 8929425). Drebrin has two isoforms produced by alternative splicing. -
Drebrin was immunoprecipitated using 0.5mg SHSY5Y whole cell extract, 5µg of Rabbit polyclonal to Drebrin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, SHSY5Y whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab60933.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 100kDa: Drebrin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab60933 has been referenced in 6 publications.
- Alfarsi LH et al. Integrated Analysis of Key Differentially Expressed Genes Identifies DBN1 as a Predictive Marker of Response to Endocrine Therapy in Luminal Breast Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32545448
- Jiang D et al. Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae. J Comp Neurol 528:729-755 (2020). PubMed: 31609468
- Elizondo DM et al. Drebrin 1 in dendritic cells regulates phagocytosis and cell surface receptor expression through recycling for efficient antigen presentation. Immunology 156:136-146 (2019). PubMed: 30317558
- Kommaddi RP et al. Aß mediates F-actin disassembly in dendritic spines leading to cognitive deficits in Alzheimer's disease. J Neurosci 38:1085-1099 (2018). PubMed: 29246925
- Wahl D et al. Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice. Cell Rep 25:2234-2243.e6 (2018). PubMed: 30463018
- Pan W et al. Effects of dihydrotestosterone on synaptic plasticity of the hippocampus in mild cognitive impairment male SAMP8 mice. Exp Ther Med 12:1455-1463 (2016). IHC-P ; Mouse . PubMed: 27588067