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    drebrin-antibody-ab60933.pdf

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Validated using a knockout cell line

Anti-Drebrin antibody (ab60933)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (6)

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Western blot - Anti-Drebrin antibody (ab60933)
  • Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab60933)
  • Western blot - Anti-Drebrin antibody (ab60933)
  • Immunoprecipitation - Anti-Drebrin antibody (ab60933)

Key features and details

  • Rabbit polyclonal to Drebrin
  • Suitable for: ICC/IF, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Drebrin antibody [EPR12634] (ab178408)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Drebrin antibody
    See all Drebrin primary antibodies
  • Description

    Rabbit polyclonal to Drebrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Quail
  • Immunogen

    Synthetic peptide corresponding to Human Drebrin aa 600 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101313)

  • Positive control

    • WB: SH-SY5Y, SK N SH and SK N BE Whole Cell Lysate, Human, Rat and Mouse Cortex Neuronal cell lysate, Rat and Mouse Hippocampus Tissue Lysate and Malme 3M Whole Cell Lysate. ICC/IF: Primary rat neurons/glia, DIV14 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab60933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 71 kDa).
IP
Use at an assay dependent concentration.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 71 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
  • Tissue specificity

    Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Contains 1 ADF-H domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q16643 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396496 Chicken
    • Entrez Gene: 505406 Cow
    • Entrez Gene: 1627 Human
    • Entrez Gene: 56320 Mouse
    • Entrez Gene: 81653 Rat
    • Omim: 126660 Human
    • SwissProt: P18302 Chicken
    • SwissProt: Q16643 Human
    • SwissProt: Q9QXS6 Mouse
    • SwissProt: Q07266 Rat
    • Unigene: 130316 Human
    • Unigene: 19016 Mouse
    • Unigene: 11247 Rat
    see all
  • Alternative names

    • D0S117E antibody
    • DBN 1 antibody
    • DBN1 antibody
    • Developmentally regulated brain protein antibody
    • Developmentally-regulated brain protein antibody
    • DKFZp434D064 antibody
    • DREB_HUMAN antibody
    • Drebrin 1 antibody
    • Drebrin antibody
    • Drebrin E antibody
    • Drebrin E2 antibody
    • Drebrin1 antibody
    • DrebrinE antibody
    see all

Images

  • Western blot - Anti-Drebrin antibody (ab60933)
    Western blot - Anti-Drebrin antibody (ab60933)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)
    Lane 4: Hu cerebellum whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab60933 observed at 100120 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab60933 was shown to specifically react with Drebrin in wild-type HAP1 cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. ab60933 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab60933)
    Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody (ab60933)

    ab60933 staining Drebrin in primary rat neurons/glia, DIV14 (prepared from E18 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDHEP) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab60933 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Western blot - Anti-Drebrin antibody (ab60933)
    Western blot - Anti-Drebrin antibody (ab60933)
    All lanes : Anti-Drebrin antibody (ab60933) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : Human Cortex Neuronal cell lysate
    Lane 5 : Rat Cortex Neuronal cell lysate
    Lane 6 : Mouse Cortex Neuronal cell lysate
    Lane 7 : Rat Hippocampus Tissue Lysate
    Lane 8 : Mouse Hippocampus Tissue Lysate
    Lane 9 : Malme 3M (Human melanoma cells) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 71 kDa
    Observed band size: 100,120 kDa why is the actual band size different from the predicted?
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    The banding pattern observed is consistent with what has been described in the literature (PMID:10234022, 8929425). Drebrin has two isoforms produced by alternative splicing.
  • Immunoprecipitation - Anti-Drebrin antibody (ab60933)
    Immunoprecipitation - Anti-Drebrin antibody (ab60933)
    Drebrin was immunoprecipitated using 0.5mg SHSY5Y whole cell extract, 5µg of Rabbit polyclonal to Drebrin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, SHSY5Y whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab60933.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 100kDa: Drebrin.

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab60933? Please let us know so that we can cite the reference in this datasheet.

ab60933 has been referenced in 6 publications.

  • Alfarsi LH  et al. Integrated Analysis of Key Differentially Expressed Genes Identifies DBN1 as a Predictive Marker of Response to Endocrine Therapy in Luminal Breast Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32545448
  • Jiang D  et al. Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae. J Comp Neurol 528:729-755 (2020). PubMed: 31609468
  • Elizondo DM  et al. Drebrin 1 in dendritic cells regulates phagocytosis and cell surface receptor expression through recycling for efficient antigen presentation. Immunology 156:136-146 (2019). PubMed: 30317558
  • Kommaddi RP  et al. Aß mediates F-actin disassembly in dendritic spines leading to cognitive deficits in Alzheimer's disease. J Neurosci 38:1085-1099 (2018). PubMed: 29246925
  • Wahl D  et al. Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice. Cell Rep 25:2234-2243.e6 (2018). PubMed: 30463018
  • Pan W  et al. Effects of dihydrotestosterone on synaptic plasticity of the hippocampus in mild cognitive impairment male SAMP8 mice. Exp Ther Med 12:1455-1463 (2016). IHC-P ; Mouse . PubMed: 27588067

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Drebrin antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Permeabilization
Yes - 0.03% TX100
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 14 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovarian carcinoma)
Specification
Ovarian carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted May 03 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Quail Tissue sections (Developing spinal cord)
Specification
Developing spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Apr 08 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 2 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Apr 06 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (E7 spinal cord)
Specification
E7 spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Apr 04 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Drebrin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Apr 01 2011

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