Product nameAnti-Drebrin antibody [EPR12634]
See all Drebrin primary antibodies
DescriptionRabbit monoclonal [EPR12634] to Drebrin
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Drebrin aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q16643
- Jurkat, PC-12, Neuro-2a, fetal brain, SH-SY5Y and HeLa lysates; SH-SY5Y cells; fetal brain lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 71 kDa).|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionDrebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
Tissue specificityBrain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 1 ADF-H domain.
- Information by UniProt
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab178408 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab178408 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab178408 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab178408 staining Drebrin in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only.
All lanes : Anti-Drebrin antibody [EPR12634] (ab178408) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : PC-12 lysate
Lane 3 : Neuro-2a lysate
Lane 4 : fetal brain lysate
Lane 5 : SH-SY5Y lysate
Lane 6 : HeLa lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71 kDa
Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation. ab178408 at 1/1000 labeling Debrin in fetal brain lysate immunoprecipitated using ab178408 at 1/10.
Immunofluorescent analysis of SH-SY5Y cells labeling Drebrin with ab178408 at 1/100.
ab178408 has not yet been referenced specifically in any publications.