Overview

  • Product name

    Anti-Drebrin antibody [EPR12634]
    See all Drebrin primary antibodies
  • Description

    Rabbit monoclonal [EPR12634] to Drebrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Drebrin aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16643

  • Positive control

    • Jurkat, PC-12, Neuro-2a, fetal brain, SH-SY5Y and HeLa lysates; SH-SY5Y cells; fetal brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 71 kDa).
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
      Lane 3: SH-SY5Y whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab178408 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab178408 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab178408 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • ab178408 staining Drebrin in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Control: PBS only.

    • All lanes : Anti-Drebrin antibody [EPR12634] (ab178408) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : PC-12 lysate
      Lane 3 : Neuro-2a lysate
      Lane 4 : fetal brain lysate
      Lane 5 : SH-SY5Y lysate
      Lane 6 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 71 kDa
      Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.

    • Immunoprecipitation. ab178408 at 1/1000 labeling Debrin in fetal brain lysate immunoprecipitated using ab178408 at 1/10.

    • Immunofluorescent analysis of SH-SY5Y cells labeling Drebrin with ab178408 at 1/100.

    References

    ab178408 has not yet been referenced specifically in any publications.

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