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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Drebrin antibody [EPR12634] (ab178408)

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Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)
  • Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)
  • Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)
  • Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (ab178408)
  • Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)
  • Anti-Drebrin antibody [EPR12634] (ab178408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12634] to Drebrin
  • Suitable for: WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Drebrin antibody [EPR12634]
    See all Drebrin primary antibodies

  • Description

    Rabbit monoclonal [EPR12634] to Drebrin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Drebrin aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16643

  • Positive control

    • Jurkat, PC-12, Neuro-2a, fetal brain, SH-SY5Y and HeLa lysates; SH-SY5Y cells; fetal brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 71 kDa).
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 71 kDa).
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

Images

  • Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)
    Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab178408 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab178408 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab178408 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)
    Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)

    ab178408 staining Drebrin in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only.

  • Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)
    Western blot - Anti-Drebrin antibody [EPR12634] (ab178408)
    All lanes : Anti-Drebrin antibody [EPR12634] (ab178408) at 1/1000 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : PC-12 lysate
    Lane 3 : Neuro-2a lysate
    Lane 4 : fetal brain lysate
    Lane 5 : SH-SY5Y lysate
    Lane 6 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 71 kDa
    Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (ab178408)
    Immunoprecipitation - Anti-Drebrin antibody [EPR12634] (ab178408)

    Immunoprecipitation. ab178408 at 1/1000 labeling Debrin in fetal brain lysate immunoprecipitated using ab178408 at 1/10.

  • Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)
    Immunocytochemistry/ Immunofluorescence - Anti-Drebrin antibody [EPR12634] (ab178408)

    Immunofluorescent analysis of SH-SY5Y cells labeling Drebrin with ab178408 at 1/100.

  • Anti-Drebrin antibody [EPR12634] (ab178408)
    Anti-Drebrin antibody [EPR12634] (ab178408)

References (0)

Publishing research using ab178408? Please let us know so that we can cite the reference in this datasheet.

ab178408 has not yet been referenced specifically in any publications.

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