Overview

  • Product name

    Anti-Drebrin antibody [EPR12640(B)]
    See all Drebrin primary antibodies
  • Description

    Rabbit monoclonal [EPR12640(B)] to Drebrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Drebrin aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16643

  • Positive control

    • SH-SY5Y, Fetal brain, HeLa and Neuro-2a lysates, SH-SY5Y cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
      Lane 3: SH-SY5Y whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab176318 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab176318 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab176318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunofluorescent staining of SH-SY5Y cells labeling Drebrin using ab176318 at a 1/100 dilution (green), with DAPI nuclear staining (blue).

    • Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate labeling Drebrin using ab176318 at a 1/10 dilution. 

    • All lanes : Anti-Drebrin antibody [EPR12640(B)] (ab176318) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : HeLa lysate
      Lane 4 : Neuro-2a lysate

      Predicted band size: 71 kDa

    References

    ab176318 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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