Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12640(B)] to Drebrin
- Suitable for: IP, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-Drebrin antibody [EPR12640(B)]
See all Drebrin primary antibodies
DescriptionRabbit monoclonal [EPR12640(B)] to Drebrin
Tested applicationsSuitable for: IP, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Drebrin aa 550 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q16643
- SH-SY5Y, Fetal brain, HeLa and Neuro-2a lysates, SH-SY5Y cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 71 kDa.|
|ICC/IF||1/100 - 1/250.|
FunctionDrebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
Tissue specificityBrain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 1 ADF-H domain.
- Information by UniProt
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab176318 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab176318 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab176318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent staining of SH-SY5Y cells labeling Drebrin using ab176318 at a 1/100 dilution (green), with DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate labeling Drebrin using ab176318 at a 1/10 dilution.
All lanes : Anti-Drebrin antibody [EPR12640(B)] (ab176318) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate
Lane 3 : HeLa lysate
Lane 4 : Neuro-2a lysate
Predicted band size: 71 kDa
ab176318 has not yet been referenced specifically in any publications.