Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)

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Western blot - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
  • Immunocytochemistry - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
  • Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
  • Western blot - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
  • Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12640(B)] to Drebrin - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free
    See all Drebrin primary antibodies

  • Description

    Rabbit monoclonal [EPR12640(B)] to Drebrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249903 is the carrier-free version of ab176318. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249903 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
Notes
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    Images

    • Western blot - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
      Western blot - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)

      This data was developed using ab176318, the same antibody clone in a different buffer formulation.

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

      Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)

      Lane 3: SH-SY5Y whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab176318 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab176318 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. ab176318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
      Immunocytochemistry - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
      This data was developed using ab176318, the same antibody clone in a different buffer formulation.Immunofluorescent staining of SH-SY5Y cells labeling Drebrin using ab176318 at a 1/100 dilution (green), with DAPI nuclear staining (blue).
    • Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
      Immunoprecipitation - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
      This data was developed using ab176318, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate labeling Drebrin using ab176318 at a 1/10 dilution.
    • Western blot - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
      Western blot - Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
      All lanes : Anti-Drebrin antibody [EPR12640(B)] (ab176318) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : Fetal brain lysate

      Predicted band size: 71 kDa



      This data was developed using ab176318, the same antibody clone in a different buffer formulation.

       

    • Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)
      Anti-Drebrin antibody [EPR12640(B)] - BSA and Azide free (ab249903)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab249903? Please let us know so that we can cite the reference in this datasheet.

    ab249903 has not yet been referenced specifically in any publications.

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