Product nameAnti-Drebrin antibody [M2F6]
See all Drebrin primary antibodies
DescriptionMouse monoclonal [M2F6] to Drebrin
SpecificityThis antibody detects Drebrin A and E in samples from human, mouse, rat, guinea pig, and cat origins and Drebrin E only in chicken samples.
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IF, IHC-FoFr, IHC-FrFl, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Guinea pig, Cat, Monkey, Quail
Chicken Drebrin E protein.
- Mouse Brain Tissue Extract (ab7934).
Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product by June 2020 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We suggest ab60933 or ab178408 or ab176318 as possible replacements.
For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituents: PBS, 50% Glycerol
Concentration information loading...
Purification notesPurified from ascites.
Our Abpromise guarantee covers the use of ab12350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 65 kDa). The difference in the size of the detected band (~110 kDa) and in the predicted (~65 kDa) is due to the large number of negatively charged residues in this protein. Block membrane with 5-10% milk for 1 hour.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration. Fix cells with 4% PFA for 15 minutes. Permeabilise cells for 15 minutes with 0.1% triton X100 in PBS (see Ikeda K et al).|
|ICC/IF||Use at an assay dependent concentration. See Toda et al.|
|IHC-FoFr||1/1000. See Keon et al.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionDrebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
Tissue specificityBrain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 1 ADF-H domain.
- Information by UniProt
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
All lanes : Anti-Drebrin antibody [M2F6] (ab12350) at 1/5000 dilution
Lanes 1-4 : Trangenic (APP/PS1) male mouse cortical lysate
Lanes 5-8 : Wild-type male mouse cortical lysate
Lane 9 : Rat cortical lysate
All lanes : Alexa-Fluor 680 conjugated goat anti-mouse IgG
Predicted band size: 65 kDa
Observed band size: 120,42,95 kDa why is the actual band size different from the predicted?
Western blot showing detection of drebrin in mouse and rat cortical lysates by ab12350.
IHC-P image of Drebrin staining on Human ovarian carcinoma sections using ab12350 (1:100). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab12350 was diluted 1:100 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab12350 for 16 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
ab12350 at 1/50 staining mouse brain tissue sections by IHC-P. The tissue fixed in 4% Formalin, diluted in Dulbecco A PBS with standard pwax processing. Citric acid 0.01M pH6 was used as antigen retrieval solution. The tissue sections were incubated with the antibody for 2 hours. A biotinylated goat polyclonal antibody was used as the secondary. The image shows the lateral ventricle showing high cytoplasmic positivity in migrating progenitor neurones in the subventricular space.There is a low level of positivity in the dendritic field of the striatum; this appears to be specific. An identical pattern of staining is seen in same area of rat brain. All non-migratory cells in the same field are negative. The Corpus Callosum is negative.
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Drebrin antibody [M2F6] (ab12350) on Quail Tissue sections (embryo d5/6 brain). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody: ab12350 incubated at 1/200 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti mouse Igs (1/200).
Immunofluorescent staining in the rat brain cerebellum with ab12350 mouse monoclonal Drebrin antibody. Drebrin-positive neurons and fibers are observed. Picture taken with X20 objective. Protocol: IHC free-floating protocol using 4% PFA fixed brain tissue. Rats were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Primary antibody ab12350 was used at 1/1000 incubated overnight at room temperature. Secondary antibody was Alexa Fluor 488 used at 1/1000, 2h incubation at room temperature. Image recoloured in Adobe photoshop.
Overlay histogram showing SH-SY5Y cells stained with ab12350 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12350, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Western blot analysis of Drebrin expression in mouse brain (1) NIH/3T3 (2), Jurkat (3), HeLa (4), and PC12 (5) using Drebrin mAb (ab12350).
This product has been referenced in:
- Zhao B et al. Microtubules Modulate F-actin Dynamics during Neuronal Polarization. Sci Rep 7:9583 (2017). Read more (PubMed: 28851982) »
- Woo JA et al. Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity. Nat Commun 8:15558 (2017). Mouse . Read more (PubMed: 28585542) »