Overview

  • Product name

    Anti-DREF antibody
  • Description

    Rabbit polyclonal to DREF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human DREF aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab48354)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab48355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable elements, but does not code for any transposase activity.
  • Tissue specificity

    Ubiquitously expressed at low levels. Expression is highest in skeletal muscle, heart, spleen and placenta.
  • Sequence similarities

    Contains 1 BED-type zinc finger.
  • Cellular localization

    Nucleus. In granular structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ac like transposable element antibody
    • ALTE antibody
    • BED type zinc finger domain containing protein 1 antibody
    • DNA replication related element binding factor antibody
    • dREF homolog antibody
    • hDREF antibody
    • KIAA0785 antibody
    • Putative Ac like transposon antibody
    • Putative Ac-like transposable element antibody
    • TRAMP antibody
    • ZBED1 antibody
    • ZBED1_HUMAN antibody
    • Zinc finger BED domain-containing protein 1 antibody
    • Zinc finger BED type containing 1 antibody
    see all

Images

  • All lanes : Anti-DREF antibody (ab48355) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 78 kDa
    Observed band size: 78 kDa
    Additional bands at: 27 kDa, 52 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab48355 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab48355, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References

This product has been referenced in:

  • Radko S  et al. Adenovirus E1A targets the DREF nuclear factor to regulate virus gene expression, DNA replication, and growth. J Virol 88:13469-81 (2014). ChIP ; Mouse . Read more (PubMed: 25210186) »
See 1 Publication for this product

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