Key features and details
- Rabbit polyclonal to DREF
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DREF antibody
See all DREF primary antibodies
DescriptionRabbit polyclonal to DREF
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Xenopus laevis
Synthetic peptide corresponding to Human DREF aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Purchase matching WB positive control:Recombinant Human DREF protein
- This antibody gave a positive signal in the following lysates: HeLa Whole Cell; HeLa Nuclear; Jurkat Whole Cell; Jurkat Nuclear; HepG2 Whole Cell; HepG2 Nuclear (Data not shown for HepG2 Whole Cell and HepG2 Nuclear lysates).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab48355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionBinds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable elements, but does not code for any transposase activity.
Tissue specificityUbiquitously expressed at low levels. Expression is highest in skeletal muscle, heart, spleen and placenta.
Sequence similaritiesContains 1 BED-type zinc finger.
Cellular localizationNucleus. In granular structures.
- Information by UniProt
- Ac like transposable element antibody
- ALTE antibody
- BED type zinc finger domain containing protein 1 antibody
All lanes : Anti-DREF antibody (ab48355) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 27 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab48355 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab48355, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab48355 has been referenced in 1 publication.
- Radko S et al. Adenovirus E1A targets the DREF nuclear factor to regulate virus gene expression, DNA replication, and growth. J Virol 88:13469-81 (2014). ChIP ; Mouse . PubMed: 25210186