Key features and details
- Rabbit polyclonal to DRIM
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DRIM antibody
DescriptionRabbit polyclonal to DRIM
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Cow, Chimpanzee, Rhesus monkey, Bat, Elephant
Synthetic peptide, corresponding to a region between residues 2735 and 2785 of human DRIM (NP_055318.2)
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86093 was affinity purified using an epitope specific to DRIM immobilized on solid support.
Our Abpromise guarantee covers the use of ab86093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 318 kDa (predicted molecular weight: 318 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceDRIM, also known as UTP20, is a component of the U3 small nucleolar RNA (snoRNA) protein complex (U3 snoRNP) and is involved in 18S rRNA processing. DRIM contains 2 HEAT repeats.
Cellular localizationNucleus; nucleolus
- 3830408P06Rik antibody
- AA617408 antibody
- Down regulated in metastasis antibody
All lanes : Anti-DRIM antibody (ab86093) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 318 kDa
Observed band size: 318 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Detection of DRIM by Western Blot of Immunprecipitate.
ab86093 at 1µg/ml staining DRIM in HeLa whole cell lysate immunoprecipitated using ab86093 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab86093 has not yet been referenced specifically in any publications.