Key features and details
- Rabbit polyclonal to DRIP130
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DRIP130 antibody
See all DRIP130 primary antibodies
DescriptionRabbit polyclonal to DRIP130
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human DRIP130 (internal sequence). The exact sequence is proprietary.
Database link: Q9ULK4
- A549 cell extracts; A549 cells; Human colon carcinoma tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, 49% PBS
'PBS is without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab196752 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab196752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/3000. Predicted molecular weight: 156 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionRequired for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.
Sequence similaritiesBelongs to the Mediator complex subunit 23 family.
- Information by UniProt
- 130 kDa transcriptional co activator antibody
- 133 kDa transcriptional co activator antibody
- Activator recruited cofactor 130 kDa component antibody
All lanes : Anti-DRIP130 antibody (ab196752) at 1/500 dilution
Lane 1 : A549 cell extracts
Lane 2 : A549 cell extracts with Immunizing peptide
Predicted band size: 156 kDa
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling DRIP130 with ab196752 at a 1/50 dilution. The image on the right was treated with the synthesized peptide.
Immunofluorescent analysis of A549 cells labeling DRIP130 with ab196752 at a 1/100 dilution. The image on the right was treated with the synthesized peptide.
ab196752 has not yet been referenced specifically in any publications.