Overview

  • Product name

    Anti-DRIP130 antibody [EPR17418] - BSA and Azide free
    See all DRIP130 primary antibodies
  • Description

    Rabbit monoclonal [EPR17418] to DRIP130 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DRIP130 aa 900-1000. The exact sequence is proprietary.
    Database link: Q9ULK4

  • Positive control

    • IHC-P: Human breast carcinoma and rat kidney tissues. ICC/IF: MCF7 cells.
  • General notes

    Ab240977 is the carrier-free version of ab200351. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240977 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 156 kDa).
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Required for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.
  • Sequence similarities

    Belongs to the Mediator complex subunit 23 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 130 kDa transcriptional co activator antibody
    • 133 kDa transcriptional co activator antibody
    • Activator recruited cofactor 130 kDa component antibody
    • Activator-recruited cofactor 130 kDa component antibody
    • ARC 130 antibody
    • ARC130 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 3 130kDa antibody
    • Cofactor required for Sp1 transcriptional activation subunit 3 130kDa mediator antibody
    • Cofactor required for Sp1 transcriptional activation subunit 3 antibody
    • CRSP 130 antibody
    • CRSP 130 kD subunit antibody
    • CRSP 133 antibody
    • CRSP 3 antibody
    • CRSP complex subunit 3 antibody
    • CRSP130 antibody
    • CRSP133 antibody
    • CRSP3 antibody
    • CRSP3 protein antibody
    • DKFZp434H0117 antibody
    • DRIP 130 antibody
    • DRIP130 antibody
    • hSur-2 antibody
    • KIAA1216 antibody
    • MED 23 antibody
    • med23 antibody
    • MED23_HUMAN antibody
    • Mediator complex subunit 23 antibody
    • Mediator of RNA polymerase II transcription subunit 23 antibody
    • MRT18 antibody
    • Protein sur-2 homolog antibody
    • SUR 2 antibody
    • SUR2 antibody
    • Transcriptional co activator CRSP 130 antibody
    • Transcriptional co activator CRSP130 antibody
    • Transcriptional coactivator CRSP130 antibody
    • Vitamin D3 receptor interacting protein antibody
    • Vitamin D3 receptor interacting protein complex 130 kDa component antibody
    • Vitamin D3 receptor-interacting protein complex 130 kDa component antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling DRIP130 with ab200351 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200351 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200351).

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling DRIP130 with ab200351 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200351).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling DRIP130 with ab200351 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200351).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab240977 has not yet been referenced specifically in any publications.

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