Anti-Drosha antibody (ab12286)
Key features and details
- Rabbit polyclonal to Drosha
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Drosha antibody
See all Drosha primary antibodies -
Description
Rabbit polyclonal to Drosha -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Drosha aa 1-100 (internal sequence).
(Peptide available asab12307) -
General notes
Three isoforms of 159, 156 and 143 kDa.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab12286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 159 kDa. | |
| ICC | Use at an assay dependent concentration. |
Target
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Function
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 2 RNase III domains. -
Domain
The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other. -
Cellular localization
Nucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. - Information by UniProt
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Database links
- Entrez Gene: 29102 Human
- Entrez Gene: 14000 Mouse
- Omim: 608828 Human
- SwissProt: Q9NRR4 Human
- SwissProt: Q5HZJ0 Mouse
- Unigene: 97997 Human
- Unigene: 293142 Mouse
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Alternative names
- DROSHA antibody
- Drosha double stranded RNA specific endoribonuclease antibody
- Drosha ribonuclease type III antibody
see all
Images
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Immunocytochemistry - Anti-Drosha antibody - ChIP Grade (ab12286)This image is courtesy of Ahmad M. Khalil and Daniel J. Driscoll, University of Florida College of Medicine Genetics Institute.Human female amniocytes immunostained with ab12286 Drosha (FITC) (1/25 dilution). The DNA is labelled red with propidium iodide. This image was submitted as part of a review by Ahmad Khalil. -
Western blot - Anti-Drosha antibody - ChIP Grade (ab12286)All lanes : Anti-Drosha antibody (ab12286) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody - ChIP Grade (ab12286)ab12286 at a 1/25 dilution staining human HeLa cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Cy3 conjugated donkey anti-rabbit polyclonal antibody.
This image is courtesy of an Abreview submitted on 7 February 2006.
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Western blot - Anti-Drosha antibody - ChIP Grade (ab12286)All lanes : Anti-Drosha antibody (ab12286) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody - ChIP Grade (ab12286)ICC/IF image of ab12286 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12286, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody - ChIP Grade (ab12286)ICC/IF image of ab12286 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12886, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (77)
ab12286 has been referenced in 77 publications.
- Pan D et al. Herpes Simplex Virus 1 Lytic Infection Blocks MicroRNA (miRNA) Biogenesis at the Stage of Nuclear Export of Pre-miRNAs. MBio 10:N/A (2019). PubMed: 30755517
- Zhang J et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun 10:1858 (2019). PubMed: 31015415
- Deng L et al. Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis. Nat Commun 10:3329 (2019). PubMed: 31350386
- Pawlicki P et al. Do G-protein coupled estrogen receptor and bisphenol A analogs influence on Leydig cell epigenetic regulation in immature boar testis ex vivo? Anim Reprod Sci 207:21-35 (2019). PubMed: 31266599
- Zhang R et al. Caloric Restriction Induces MicroRNAs to Improve Mitochondrial Proteostasis. iScience 17:155-166 (2019). PubMed: 31279933
