Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Brain Day 25)
Specification
Mouse Brain Day 25
Fixative
neutral buffered formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - TBS-Tween (0.1%)
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS- 0.3% Triton X and 10% serum
Secondary antibody
Name
Non-Abcam antibody was used: Vector Biotinylated anti-rabbit IgG (H+L) Conjugat
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Neurons in the day 25 old female mouse brain. Used ab12286 at 1/100 staining mouse brain tissue sections by IHC-P. Expression was noted in various neurons of the brain and in the Cerebellum shown here. Antibody optimization included dilutions of 1:25, 1:100, 1:200, 1:500. The tissue was neutral buffered formalin fixed for 24hours at 4C before embedding in paraffin. Sections were blocked with serum prior to incubation with the antibody overnight at 4C. A biotinylated goat anti-rabbit IgG was used as the secondary.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kai Orton
Verified customer
Submitted May 28 2012