Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (6%)
Sample
Human Cell lysate - whole cell (human osteosarcoma cell line U2OS)
Specification
human osteosarcoma cell line U2OS
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Li-Cor Goat anti rabbit antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
Li-Cor Odyssey scanner
Bands
Specific: 150 kDa Non-specific: 250, 150, 130, 100, 80, 7 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Lane 1 : Control siRNA
Lane 2: Drosha siRNA
Abcam response
Thank you for submitting this Abreview. We recommend incubating the primary antibody for 12 hours at 4 degrees to reduce the background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted May 21 2013