Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (13.5%)
Sample
Mouse Cell lysate - whole cell (Mouse N2A neuroblastoma)
Specification
Mouse N2A neuroblastoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
Li-Cor Odyssey Scanner
Bands
Specific: 150, 158 kDa Non-specific: 60, 120, 140, 230 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted May 27 2013