Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - nuclear (A549)
Specification
A549
Total protein in input
25 µg
Other product details
Concentration
10 µg/mg lysate
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
CoIP showed weak Drosha signal, probably due to reused WB antibody.
Drosha coip with its binding parter DGCR8 successfully.
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Submitted Feb 20 2015