Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293)
Loading amount
20 µg
Specification
HEK-293
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5 %
Other product details
Concentration
1 mg/ml
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 171 kDa
Positive control
Actin
Negative control
No negative control was used in this experiment
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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Submitted Jan 12 2007