Overview

  • Product name
  • Description
    Goat polyclonal to Drosha
  • Host species
    Goat
  • Specificity
    This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide:

    C-KQTDKQKLAQRE

    , corresponding to internal sequence amino acids 811-822 of Human Drosha

  • Positive control
    • Human Liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 159 kDa).

Target

  • Function
    Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 2 RNase III domains.
  • Domain
    The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • DROSHA antibody
    • Drosha double stranded RNA specific endoribonuclease antibody
    • Drosha ribonuclease type III antibody
    • Etohi2 antibody
    • HSA242976 antibody
    • Nuclear RNase III Drosha antibody
    • p241 antibody
    • Protein Drosha antibody
    • Putative protein p241 which interacts with transcription factor Sp1 antibody
    • Putative ribonuclease III antibody
    • RANSE3L antibody
    • Ribonuclease 3 antibody
    • Ribonuclease III antibody
    • Ribonuclease III nuclear antibody
    • Ribonuclease type III nuclear antibody
    • RibonucleaseIII antibody
    • RN 3 antibody
    • RN3 antibody
    • RNase 3 antibody
    • RNase III antibody
    • RNase3 antibody
    • RNASE3L antibody
    • RNaseIII antibody
    • RNASEN antibody
    • RNC_HUMAN antibody
    see all

Images

  • Anti-Drosha antibody (ab58589) at 0.5 µg/ml (incubated for 1 hour) + Human Liver lysate (35µg protein in RIPA buffer).

    Developed using the ECL technique.

    Predicted band size: 159 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • IHC image of ab58589 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58589, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
See all 3 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Answer

Thank you for submitting an Abreview of ab58589. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested application, we would like to follow up on this.
Based on the information in your review, could you please provide the following additional information:
- Could you please specify which antibody was used for WB? Was is also ab58589 or a different Abcam antibody?
- Also could you please provide a legend for the WB/IP image on the Abreview?
You can either add this information on the review, or just send it to me and I'd be happy to add it for you - whichever way is easier for you.
In terms of improving the results you had obtained the following tips might be of help:
1) incubate samples with the IP antibody overnight at 4 degree.
2) try various antibody to lysate ratios to find the optimal one for this antibody and your samples (e.g. 1/100, 1/250, 1/500, 1/750 and 1/1000).
If you decide to follow these suggestions, please let me know if they are helpful.
I look forward to your reply.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS)
Total protein in input
2 µg
Specification
U2OS
Treatment
+/- UVC
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

Submitted May 25 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab58589. Please see below for the discount code for testing this antibody in IP.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee (for WB, human). Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab58589 in IP. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IPand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

I wish you the best of luck with your research.

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Answer

Thank you for your response.
ICC stands for for immuno-cytochemistry which requires cells (not tissues); whilst IHC is the abbreviation for immuno-histochemistry.
If you wish to use tissue sections of human gastric samples then you need an antibody which is suitable for immuno-histochemistry (IHC). I assume the samples are fixed in formalin and embedded in paraffin so you are looking for antibodies particularly for IHC-P? I can confirm that ab58589 and ab85027 both have been characterized for this type of immunoassay.
Please take a look at the on-line product datasheets to see which one would suit your need best. Your choice/decision may depend on the detection system/ sample type you wish to apply etc.
I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Answer

Thank you for your response.
Both antibodies (ab58589 and ab85027) are suitable for IHC-P application and they can detect human Drosha. I would suggest taking a look at the on-line product datasheets to see which one would suit your need best. Your choice/decision may depend on the detection system/ sample type you wish to apply etc.
I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Answer

Thank you for your enquiry regarding ab12286.
Currently, we have 12 specific publications on this product (ab12286) and the list of these papers can be viewed on the on the on-line datasheet. Since ab12286 has been tested in ICC/IF, IP, WB, ICC, ChIP; most of these articles are WB-related. We do not have any data/publication which would support its suitability for IHC since it has not been characterized yet.
The other two antibodies against Drosha (ab58589 and ab85027) have been tested in IHC-P and we guarantee them in this application but not aware of any IHC-specific published work yet. As soon as we have further information, we will update the datasheets accordingly.
I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

Question
Answer


I am very pleased to hear you would like to accept our offer and test ab12286 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the "Additional Comments" section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your response.

I am happy to hear that you would like to take advantage of our 100% Abreview testing discount to test ab12286 in IHC. In order for me to process the discount code I will need a little information. First, I realize that you will be testing in IHC but could you please clarify which specific IHC process you will be using, e.g paraffin enbedded, frozen tissue, etc.? Second, would you please let me know what the species is that you will be testing ab12286 on?

Thank you for your help. Please let me know if you have any questions.

Read More

Answer

Thank you for contacting us.

That product is guaranteed to work in WB in human or mouse tissue. IHC is not guaranteed as we have not tested it yet. If you are interested in testing this, it may qualify for our 100% abreview discount. This is a program in which you purchase the product. Test and review it and then recieve a free priamry antibody. Tio qualify for this you would have to test in mouse, human or another species that has at least 85% homology with human Drosha. Please let me know if you are interested and I will send you further information.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

The product that you have expressed interest in is covered by our Abpromise guarantee. We promise to provide support, replacement or refund of the antibody if you use it according to the information on our datasheet. For this particular product we guarantee that it will work in IHC-P and WB in rat, human, mouse and dog. More information about our Abpromise may be found at the link below:

https://www.abcam.com/index.html?pageconfig=abpromise


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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