Product nameAnti-Drosha antibody
See all Drosha primary antibodies
DescriptionGoat polyclonal to Drosha
SpecificityThis antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Dog
- Human Liver lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab58589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 159 kDa).
1 hour primary incubation is recommended for this product.
FunctionRibonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
Sequence similaritiesContains 1 DRBM (double-stranded RNA-binding) domain.
Contains 2 RNase III domains.
DomainThe 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
Cellular localizationNucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
- Information by UniProt
- DROSHA antibody
- Drosha double stranded RNA specific endoribonuclease antibody
- Drosha ribonuclease type III antibody
Anti-Drosha antibody (ab58589) at 0.5 µg/ml (incubated for 1 hour) + Human Liver lysate (35µg protein in RIPA buffer).
Developed using the ECL technique.
Predicted band size: 159 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
IHC image of ab58589 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58589, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab58589 has been referenced in 3 publications.
- Wei YB et al. Elevation of Il6 is associated with disturbed let-7 biogenesis in a genetic model of depression. Transl Psychiatry 6:e869 (2016). PubMed: 27529677
- Adam O et al. Role of miR-21 in the pathogenesis of atrial fibrosis. Basic Res Cardiol 107:278 (2012). WB ; Rat . PubMed: 22760500
- Kye MJ et al. NMDA Mediated Contextual Conditioning Changes miRNA Expression. PLoS One 6:e24682 (2011). WB ; Rat . PubMed: 21931811