Anti-DRP1 antibody [3B5] (ab56788)
![Immunoprecipitation - Anti-DRP1 antibody [3B5] (ab56788)](https://www.abcam.com/ps/products/56/ab56788/Images/DNM1L-Primary-antibodies-ab56788-37.jpg "Immunoprecipitation - Anti-DRP1 antibody [3B5] (ab56788)")
Key features and details
- Mouse monoclonal [3B5] to DRP1
- Suitable for: IHC-P, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-DRP1 antibody [3B5]
See all DRP1 primary antibodies -
Description
Mouse monoclonal [3B5] to DRP1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human DRP1 aa 1-710.
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General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
3B5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
Use at an assay dependent concentration.
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| IP | (2) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed. -
Tissue specificity
Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. -
Involvement in disease
Note=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage. -
Sequence similarities
Belongs to the dynamin family.
Contains 1 GED domain. -
Domain
The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization. -
Post-translational
modificationsPhosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
Ubiquitination by MARCH5 affects mitochondrial morphology. -
Cellular localization
Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 10059 Human
- Omim: 603850 Human
- SwissProt: O00429 Human
- Unigene: 556296 Human
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Alternative names
- DLP1 antibody
- dnm1l antibody
- DNM1L_HUMAN antibody
see all
Images
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Immunoprecipitation - Anti-DRP1 antibody [3B5] (ab56788)
DNM1L was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Mouse monoclonal to DNM1L and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56788.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 90kDa; DNM1L.This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [3B5] (ab56788)](https://www.abcam.com/ps/products/56/ab56788/Images/ab56788_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [3B5] (ab56788)DNM1L antibody (ab56788) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human leiomyosarcoma tissue.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-DRP1 antibody [3B5] (ab56788)](https://www.abcam.com/ps/products/56/ab56788/Images/DNM1L-Primary-antibodies-ab56788-1.jpg)
Flow Cytometry - Anti-DRP1 antibody [3B5] (ab56788)Overlay histogram showing HEK293 cells stained with ab56788 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56788, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (162)
ab56788 has been referenced in 162 publications.
- Lee JM et al. Mitochondrial Transplantation Modulates Inflammation and Apoptosis, Alleviating Tendinopathy Both In Vivo and In Vitro. Antioxidants (Basel) 10:N/A (2021). PubMed: 33925007
- Tian F & Zhang Y Overexpression of SERCA2a Alleviates Cardiac Microvascular Ischemic Injury by Suppressing Mfn2-Mediated ER/Mitochondrial Calcium Tethering. Front Cell Dev Biol 9:636553 (2021). PubMed: 33869181
- Zhang Y et al. Mdivi-1 alleviates brain damage and synaptic dysfunction after intracerebral hemorrhage in mice. Exp Brain Res 239:1581-1593 (2021). PubMed: 33754161
- Simpson CL et al. NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation. Cell Rep 34:108689 (2021). PubMed: 33535046
- Wang D et al. FGF1?HBS prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppression. Signal Transduct Target Ther 6:133 (2021). PubMed: 33762571
