Overview

  • Product name
    Anti-DRP1 antibody [OTI4F6]
    See all DRP1 primary antibodies
  • Description
    Mouse monoclonal [OTI4F6] to DRP1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-736 of Human DRP1 produced in HEK293T cells (NP_036192).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNA; HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cell extracts; Human lung carcinoma, ovary adenocarcinoma, pancreas, pancreas carcinoma and endometrium tissues.
  • General notes

    Dilute in PBS (pH7.3) before use.

    The clone number has been updated from 4F6 to OTI4F6, both clone numbers name the same clone.

    This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab156951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/4000. Predicted molecular weight: 82 kDa.
IHC-P 1/150.

Target

  • Function
    Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
    Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.
  • Tissue specificity
    Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
  • Involvement in disease
    Note=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.
  • Sequence similarities
    Belongs to the dynamin family.
    Contains 1 GED domain.
  • Domain
    The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.
  • Post-translational
    modifications
    Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
    Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
    S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
    Ubiquitination by MARCH5 affects mitochondrial morphology.
  • Cellular localization
    Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • DLP1 antibody
    • dnm1l antibody
    • DNM1L_HUMAN antibody
    • Dnm1p/Vps1p-like protein antibody
    • dnml1 antibody
    • DRP1 antibody
    • DVLP antibody
    • Dymple antibody
    • Dynamin 1 like antibody
    • Dynamin family member proline-rich carboxyl-terminal domain less antibody
    • Dynamin like protein antibody
    • Dynamin related protein 1 antibody
    • Dynamin-1-like protein antibody
    • Dynamin-like protein 4 antibody
    • Dynamin-like protein antibody
    • Dynamin-like protein IV antibody
    • Dynamin-related protein 1 antibody
    • DYNIV 11 antibody
    • EMPF antibody
    • EMPF1 antibody
    • FLJ41912 antibody
    • HdynIV antibody
    • VPS1 antibody
    see all

Images

  • All lanes : Anti-DRP1 antibody [OTI4F6] (ab156951) at 1/200 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 82 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC200418
  • All lanes : Anti-DRP1 antibody [OTI4F6] (ab156951) at 1/200 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 82 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC200418
  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
  • Immunohistochemical analysis of paraffin-embedded Human ovary adenocarcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DRP1 with ab156951 at 1/150 dilution
  • Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
  • Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling DRP1 with ab156951 at 1/150 dilution

References

This product has been referenced in:
  • Yang S  et al. Mitochondrial dysfunction driven by the LRRK2-mediated pathway is associated with loss of Purkinje cells and motor coordination deficits in diabetic rat model. Cell Death Dis 5:e1217 (2014). WB ; Rat . Read more (PubMed: 24810053) »

See 1 Publication for this product

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