Anti-DRP1 (phospho S637) antibody (ab193216)
Key features and details
- Rabbit polyclonal to DRP1 (phospho S637)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DRP1 (phospho S637) antibody
See all DRP1 primary antibodies -
Description
Rabbit polyclonal to DRP1 (phospho S637) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human DRP1 (phospho S637). The exact sequence is proprietary. NCBI Protein#: NP_036192.2.
Database link: O00429 -
Positive control
- WB: Jurkat and K562 cell extracts; 293 whole cell lysates
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab193216 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/500 - 1/1000. Predicted molecular weight: 82 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 82 kDa. |
Target
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Function
Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed. -
Tissue specificity
Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. -
Involvement in disease
Note=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage. -
Sequence similarities
Belongs to the dynamin family.
Contains 1 GED domain. -
Domain
The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization. -
Post-translational
modificationsPhosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
Ubiquitination by MARCH5 affects mitochondrial morphology. -
Cellular localization
Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 10059 Human
- Entrez Gene: 74006 Mouse
- Entrez Gene: 114114 Rat
- Omim: 603850 Human
- SwissProt: O00429 Human
- SwissProt: Q8K1M6 Mouse
- SwissProt: O35303 Rat
- Unigene: 556296 Human
see all -
Alternative names
- DLP1 antibody
- dnm1l antibody
- DNM1L_HUMAN antibody
see all
Images
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All lanes : Anti-DRP1 (phospho S637) antibody (ab193216) at 1/500 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : Hela whole cell lysate treated with 100nM Calyculin A for 30min
Lane 3 : Hela whole cell lysate treated with 10 µM Forskolin for 30min
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 30 secondsBlocking and diluting buffer and concentration: 5% TBST
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All lanes : Anti-DRP1 (phospho S637) antibody (ab193216)
Lane 1 : 293 whole cell lysates with phospho-blocking peptide (P-Peptide) treatment
Lane 2 : 293 whole cell lysates with non-phospho-blocking peptide (N-Peptide) treatment
Lane 3 : 293 whole cell lysates with no treatment
Predicted band size: 82 kDa -
All lanes : Anti-DRP1 (phospho S637) antibody (ab193216) at 1/500 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract with antigen-specific peptide
Predicted band size: 82 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab193216 has been referenced in 26 publications.
- He C et al. Recurrent moderate hypoglycemia accelerates the progression of Alzheimer's disease through impairment of the TRPC6/GLUT3 pathway. JCI Insight 7:N/A (2022). PubMed: 35077394
- Jaroslawska J et al. Increasing energy expenditure through exercise and low ambient temperature offers oxidative protection to the hypothalamus after high-fat feeding to mice. J Neuroendocrinol 34:e13095 (2022). PubMed: 35138671
- Zhang Y et al. Drp1 Regulated Mitochondrial Hypofission Promotes the Invasion and Proliferation of Growth Hormone-Secreting Pituitary Adenomas via Activating STAT3. Front Oncol 12:739631 (2022). PubMed: 35463323
- Li J et al. Calenduloside E alleviates cerebral ischemia/reperfusion injury by preserving mitochondrial function. J Mol Histol 53:713-727 (2022). PubMed: 35819738
- Pang Y et al. HIGD-1B inhibits hypoxia-induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Mol Med Rep 24:N/A (2021). PubMed: 34080026