Key features and details
- Rabbit polyclonal to DRP1 (phospho S637)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DRP1 (phospho S637) antibody
See all DRP1 primary antibodies
DescriptionRabbit polyclonal to DRP1 (phospho S637)
Specificityab193216 detects endogenous levels of DRP1 only when phosphorylated at Serine 637.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human DRP1 (phospho S637). The exact sequence is proprietary. NCBI Protein#: NP_036192.2.
Database link: O00429
- WB: Jurkat and K562 cell extracts; 293 whole cell lysates
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab193216 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab193216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 82 kDa.|
FunctionFunctions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.
Tissue specificityUbiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
Involvement in diseaseNote=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.
Sequence similaritiesBelongs to the dynamin family.
Contains 1 GED domain.
DomainThe GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.
modificationsPhosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
Ubiquitination by MARCH5 affects mitochondrial morphology.
Cellular localizationCytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
- Information by UniProt
- DLP1 antibody
- dnm1l antibody
- DNM1L_HUMAN antibody
All lanes : Anti-DRP1 (phospho S637) antibody (ab193216)
Lane 1 : 293 whole cell lysates with phospho-blocking peptide (P-Peptide) treatment
Lane 2 : 293 whole cell lysates with non-phospho-blocking peptide (N-Peptide) treatment
Lane 3 : 293 whole cell lysates with no treatment
Predicted band size: 82 kDa
All lanes : Anti-DRP1 (phospho S637) antibody (ab193216) at 1/500 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract with antigen-specific peptide
Predicted band size: 82 kDa
ab193216 has been referenced in 10 publications.
- Yu B et al. Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics. Nat Commun 11:2549 (2020). PubMed: 32439975
- Huang Z et al. Renalase attenuates mitochondrial fission in cisplatin-induced acute kidney injury via modulating sirtuin-3. Life Sci 222:78-87 (2019). PubMed: 30797821
- Wang Y et al. Necrostatin-1 ameliorates the pathogenesis of experimental autoimmune encephalomyelitis by suppressing apoptosis and necroptosis of oligodendrocyte precursor cells. Exp Ther Med 18:4113-4119 (2019). PubMed: 31611942
- Long RT et al. Augmenter of Liver Regeneration Alleviates Renal Hypoxia-Reoxygenation Injury by Regulating Mitochondrial Dynamics in Renal Tubular Epithelial Cells. Mol Cells 42:893-905 (2019). PubMed: 31822044
- Wang H et al. Zearalenone regulates endometrial stromal cell apoptosis and migration via the promotion of mitochondrial fission by activation of the JNK/Drp1 pathway. Mol Med Rep 17:7797-7806 (2018). PubMed: 29620184
- Gong Y et al. Inactivation of glycogen synthase kinase-3a is required for mitochondria-mediated apoptotic germ cell phagocytosis in Sertoli cells. Aging (Albany NY) 10:3104-3116 (2018). PubMed: 30398976
- Yang YL et al. Ginsenoside Rg5 increases cardiomyocyte resistance to ischemic injury through regulation of mitochondrial hexokinase-II and dynamin-related protein 1. Cell Death Dis 8:e2625 (2017). PubMed: 28230856
- Zhou H et al. Empagliflozin rescues diabetic myocardial microvascular injury via AMPK-mediated inhibition of mitochondrial fission. Redox Biol 15:335-346 (2017). PubMed: 29306791
- Cowan DB et al. Transit and integration of extracellular mitochondria in human heart cells. Sci Rep 7:17450 (2017). PubMed: 29234096
- Chen Z et al. Hyperoside alleviates adriamycin-induced podocyte injury via inhibiting mitochondrial fission. Oncotarget 8:88792-88803 (2017). PubMed: 29179476