Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156)](https://www.abcam.com/ps/products/27/ab27156/Images/ab27156-448957-antidsdnaantibodyimmunohistochemistryskinhuman.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156)")
Key features and details
- Mouse monoclonal [35I9 DNA] to ds DNA - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Species independent
- Isotype: IgG2a
Overview
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Product name
Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free
See all ds DNA primary antibodies -
Description
Mouse monoclonal [35I9 DNA] to ds DNA - BSA and Azide free -
Host species
Mouse -
Specificity
Primarily Double stranded DNA. Measurements by immuno-CE yielded KD's of 0.71 µM and 0.09 µM, for the interaction of this antibody with ss- and dsDNA, respectively. Strong reactivity with both ss- and dsDNA has been observed on dotblots as well as very weak reactivity with RNA. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- IHC-P: human skin tissue sections.
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General notes
This antibody has been shown to be useful, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength.
Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113).
See other anti-mouse secondary antibodies that can be used with this antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
This antibody has been shown to be useful, using immunofluorescence, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength. -
Clonality
Monoclonal -
Clone number
35I9 DNA -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs. -
Cellular localization
Nuclear -
Alternative names
- Double stranded deoxyribonucleic acid antibody
- Double stranded DNA antibody
- dsDNA antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (ab27156)
IHC image of ds DNA staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab27156, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (71)
ab27156 has been referenced in 71 publications.
- Shen JZ et al. FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells. Cell 184:352-369.e23 (2021). PubMed: 33357448
- Sercel AJ et al. Stable transplantation of human mitochondrial DNA by high-throughput, pressurized isolated mitochondrial delivery. Elife 10:N/A (2021). PubMed: 33438576
- Hayden H et al. ELISA detection of MPO-DNA complexes in human plasma is error-prone and yields limited information on neutrophil extracellular traps formed in vivo. PLoS One 16:e0250265 (2021). PubMed: 33886636
- Sato A et al. Immunofluorescence microscopy-based assessment of cytosolic DNA accumulation in mammalian cells. STAR Protoc 2:100488 (2021). PubMed: 34041502
- Wan L et al. Translation stress and collided ribosomes are co-activators of cGAS. Mol Cell 81:2808-2822.e10 (2021). PubMed: 34111399
