Key features and details
- Rabbit polyclonal to Dscam
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Product nameAnti-Dscam antibody
See all Dscam primary antibodies
DescriptionRabbit polyclonal to Dscam
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Drosophila melanogaster
Synthetic peptide corresponding to Drosophila melanogaster Dscam aa 1150-1250 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Drosophila S2 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab43847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 222 kDa.|
FunctionCell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38.
Tissue specificityPrimarily expressed in brain.
Sequence similaritiesContains 6 fibronectin type-III domains.
Contains 10 Ig-like C2-type (immunoglobulin-like) domains.
DomainIg-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC.
modificationsPhosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin.
Cellular localizationSecreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons.
- Information by UniProt
- GenBank: AF260530 Drosophila melanogaster
- CHD2 42 antibody
- CHD2 52 antibody
- CHD2 antibody
Anti-Dscam antibody (ab43847) at 1 µg/ml + Drosophila S2 Cells at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 222 kDa
Observed band size: 235 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab43847 has been referenced in 1 publication.
- Jin XK et al. Immunoglobulin superfamily protein Dscam exhibited molecular diversity by alternative splicing in hemocytes of crustacean, Eriocheir sinensis. Fish Shellfish Immunol 35:900-9 (2013). PubMed: 23856639