Overview

  • Product name

  • Description

    Rabbit polyclonal to Dscam
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Drosophila melanogaster Dscam aa 1150-1250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab43846)

  • Positive control

    • This antibody gave a positive signal in Drosophila S2 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 222 kDa.

Target

  • Function

    Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38.
  • Tissue specificity

    Primarily expressed in brain.
  • Sequence similarities

    Contains 6 fibronectin type-III domains.
    Contains 10 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Ig-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC.
  • Post-translational
    modifications

    Phosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin.
  • Cellular localization

    Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons.
  • Information by UniProt
  • Database links

    • Alternative names

      • CHD2 42 antibody
      • CHD2 52 antibody
      • CHD2 antibody
      • Down syndrome cell adhesion molecule antibody
      • DSCAM antibody
      • DSCAM_HUMAN antibody
      • human CHD2-52 down syndrome cell adhesion molecule 2, 9 antibody
      • OTTHUMP00000109193 2 antibody
      • SD11109p antibody
      see all

    Images

    • Anti-Dscam antibody (ab43847) at 1 µg/ml + Drosophila S2 Cells at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 222 kDa
      Observed band size: 235 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 minute

    References

    This product has been referenced in:

    • Jin XK  et al. Immunoglobulin superfamily protein Dscam exhibited molecular diversity by alternative splicing in hemocytes of crustacean, Eriocheir sinensis. Fish Shellfish Immunol 35:900-9 (2013). Read more (PubMed: 23856639) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (fly heads and embryos)
    Loading amount
    10 µg
    Specification
    fly heads and embryos
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Apr 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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