Anti-Dscam antibody (ab51974)
Key features and details
- Goat polyclonal to Dscam
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Dscam antibody
See all Dscam primary antibodies -
Description
Goat polyclonal to Dscam -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog -
Immunogen
Peptide with sequence CDSWDSAQRTKDVSPQ, from the internal region of the protein sequence (Human)
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Positive control
- NIH/3T3 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 170 kDa.
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 170 kDa. A 1 hour primary incubation is recommended for this product. |
Target
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Function
Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. -
Tissue specificity
Primarily expressed in brain. -
Sequence similarities
Contains 6 fibronectin type-III domains.
Contains 10 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Ig-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC. -
Post-translational
modificationsPhosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin. -
Cellular localization
Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons. - Information by UniProt
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Database links
- Entrez Gene: 1826 Human
- Entrez Gene: 13508 Mouse
- Entrez Gene: 171119 Rat
- Omim: 602523 Human
- SwissProt: O60469 Human
- SwissProt: Q9ERC8 Mouse
- SwissProt: Q8VHZ8 Rat
- Unigene: 397800 Human
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Alternative names
- CHD2 42 antibody
- CHD2 52 antibody
- CHD2 antibody
see all
Images
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Anti-Dscam antibody (ab51974) at 1 µg/ml + NIH/3T3 cell lysate in RIPA buffer at 35 µg
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Anti-Dscam antibody (ab51974) at 2 µg/ml + Human Brain (Cerebellum) lysate in RIPA buffer at 35 µg
Observed band size: 170 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (0)
ab51974 has not yet been referenced specifically in any publications.