Anti-DCC1 antibody (ab168133)
Key features and details
- Mouse polyclonal to DCC1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DCC1 antibody
See all DCC1 primary antibodies -
Description
Mouse polyclonal to DCC1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant full length protein within Human DCC1 aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_076999.1 -
Positive control
- DCC1 transfected 293T cell lysate; A431 cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Loads PCNA onto primed templates regulating velocity, spacing and restart activity of replication forks. May couple DNA replication to sister chromatid cohesion through regulation of the acetylation of the cohesin subunit SMC3. -
Sequence similarities
Belongs to the DCC1 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79075 Human
- Entrez Gene: 72107 Mouse
- Omim: 613203 Human
- SwissProt: Q9BVC3 Human
- SwissProt: Q14AI0 Mouse
- Unigene: 315167 Human
- Unigene: 277084 Mouse
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Alternative names
- DCC1 antibody
- DCC1_HUMAN antibody
- Defective in sister chromatid cohesion 1 homolog antibody
see all
Images
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Anti-DCC1 antibody (ab168133) at 1 µg/ml + A431 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 45 kDa -
All lanes : Anti-DCC1 antibody (ab168133) at 1 µg/ml
Lane 1 : DCC1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 45 kDa -
Immunofluorescence analysis of HeLa cells labeling DCC1 with ab168133 at 10 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab168133 has not yet been referenced specifically in any publications.