Overview

  • Product name

    Anti-DSTN antibody [EPR15828]
    See all DSTN primary antibodies
  • Description

    Rabbit monoclonal [EPR15828] to DSTN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human DSTN aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P60981

  • Positive control

    • MCF-7, HepG2, HeLa and A549 cell lysate. Human cervical carcinoma tissue. MCF-7 cells.
  • General notes

     

     

    Previously labelled as Destrin. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15828
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/50.
IP 1/30.

Target

  • Function

    Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
  • Tissue specificity

    Widely distributed in various tissues.
  • Sequence similarities

    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications

    ISGylated.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610043P17Rik antibody
    • ACTDP antibody
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AU042046 antibody
    • bA462D18.2 antibody
    • corn1 antibody
    • DEST_HUMAN antibody
    • Destrin (actin depolymerizing factor) antibody
    • Destrin antibody
    • DSN antibody
    • Dstn antibody
    • Epididymis luminal protein 32 antibody
    • HEL32 antibody
    • Sid 23 antibody
    • sid23p antibody
    see all

Images

  • All lanes : Anti-DSTN antibody [EPR15828] (ab192262) at 1/5000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

  • Immunofluorescent analysis of 4% formaldehyde fixed MCF-7 cells labeling DSTN using ab192262 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. The two negative controls; 1. Primary ab concentration (anti-DSTN) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-DSTN) is 1:50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.

  • Immunohistochemical analysis of paraffin embedded human cervical carcinoma  tissue sections labeling DSTN using ab192262 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Lysate from A549 cells was immunoprecipitated with ab192262 at a 1/30 dilution. For the subsequent blot, ab192262 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.

References

ab192262 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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