Key features and details
- Rabbit polyclonal to DTD1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DTD1 antibody
See all DTD1 primary antibodies
DescriptionRabbit polyclonal to DTD1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab200559 was affinity purified using an epitope specific to DTD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab200559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 23 kDa.|
FunctionHydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine.
Tissue specificityExpressed in many adult and fetal tissues. Highest levels in testis, ovary, spleen and in adult and fetal brain.
Sequence similaritiesBelongs to the DTD family.
- Information by UniProt
- bA379J5.3 antibody
- bA555E18.1 antibody
- C20orf88 antibody
All lanes : Anti-DTD1 antibody (ab200559) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 23 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Detection of DTD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab200559 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab200559 has not yet been referenced specifically in any publications.