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DTT Reducer (10X) - RunBlue™(ab119199) Go to datasheet (ab119199)
I tested four samples and one control (HeLa RIPA lysate). Loading buffer has DTT (100mM final) and SDS (2% final). Samples are boiled. I guess I was wondering whether you guys have tested the dimerization of MCTP1 (if that's not possible, whether certain reducing reagent is better than others). I am planning to knock down this protein with siRNA to test which bandcorrespondsto MCTP1.
Asked on Aug 09 2012
Thank you for your reply.
I have not seen that we have tested or have any additional data regarding the dimerization of this protein or which reducing agents might be more effective. We generally use a 2x Laemmli buffer with 4% SDS, or use our Optiblot systems when testing. I have place both the recipe for the standard 2x Laemmli buffer and links to our Optiblot page and a few recommended products below.
0.004% bromophenol blue
0.125 M Tris-HCl
Check the pH and adjust pH to 6.8.
We currently are running an August promotion for 25% off items in our Optiblot range by using the code NEW-T7193 when ordering by 31 August 2012.
This is the link to our entire range:
I have added a few other links which are relevant:
I also wanted to remind you that this product is covered by our Abpromise guarantee. If this product does not perform as stated on our datasheet and is a product purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to the specifications listed on our datasheet.
I hope this information is helpful to you and look forward to hearing the results of your siRNA experiment. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Aug 09 2012